Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26739 | 3' | -52.2 | NC_005808.1 | + | 7142 | 0.7 | 0.626575 |
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Target: 5'- aGUCGUcGGCugcgGCCUGGAUGaccaGGGCCGg -3' miRNA: 3'- gCAGCAaCUG----CGGGCUUAUg---UCUGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 19607 | 0.7 | 0.626575 |
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Target: 5'- gGUCGaucUUGcCGUCCGGggucAUGCGGGCCGc -3' miRNA: 3'- gCAGC---AACuGCGGGCU----UAUGUCUGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 17166 | 0.7 | 0.626575 |
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Target: 5'- aCGUCGUcGauGCGCCCGAAcauuucCGGGCUGa -3' miRNA: 3'- -GCAGCAaC--UGCGGGCUUau----GUCUGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 6426 | 0.7 | 0.604033 |
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Target: 5'- uGUCGUUGGCGCCUGuc-ACGGuCUu -3' miRNA: 3'- gCAGCAACUGCGGGCuuaUGUCuGGc -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 39852 | 0.71 | 0.537309 |
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Target: 5'- gGUCGgc-ACGCCCGA--GCAGcGCCGg -3' miRNA: 3'- gCAGCaacUGCGGGCUuaUGUC-UGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 12447 | 0.75 | 0.358349 |
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Target: 5'- uGcCGcUGAUGUCCGAcgcgGUGCAGGCCGg -3' miRNA: 3'- gCaGCaACUGCGGGCU----UAUGUCUGGC- -5' |
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| 26739 | 3' | -52.2 | NC_005808.1 | + | 7387 | 1.11 | 0.001346 |
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Target: 5'- aCGUCGUUGACGCCCGAAUACAGACCGg -3' miRNA: 3'- -GCAGCAACUGCGGGCUUAUGUCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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