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miRNA: 3'- gCGGCCGC-AGGUGgGaa-GCGGCGGGu -5'
miRNA: 3'- gCGGCCGCAGGug-GgaaGCGGCGGGU- -5'
miRNA: 3'- -GCGGCCGCAGGUGGGAAGCGGCGGGU- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 26740 | 5' | -64.2 | NC_005808.1 | + | 34702 | 0.73 | 0.078455 |
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Target: 5'- aGCUGGCGaUCUACgCacgCGCCGCCCu -3' miRNA: 3'- gCGGCCGC-AGGUGgGaa-GCGGCGGGu -5' |
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| 26740 | 5' | -64.2 | NC_005808.1 | + | 32330 | 0.78 | 0.032145 |
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Target: 5'- gGCCGGCGUCCcgaCaacCGCCGCCCAg -3' miRNA: 3'- gCGGCCGCAGGug-GgaaGCGGCGGGU- -5' |
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| 26740 | 5' | -64.2 | NC_005808.1 | + | 7784 | 1.08 | 0.000138 |
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Target: 5'- gCGCCGGCGUCCACCCUUCGCCGCCCAg -3' miRNA: 3'- -GCGGCCGCAGGUGGGAAGCGGCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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