Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26744 | 3' | -53.2 | NC_005808.1 | + | 1463 | 0.66 | 0.778424 |
Target: 5'- gGCGCGGUCggCGGCcuccuGGCAGgccGGGGUa- -3' miRNA: 3'- -UGUGCUAGuaGUUG-----CCGUC---UCCCAgc -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 15233 | 1.1 | 0.001075 |
Target: 5'- gACACGAUCAUCAACGGCAGAGGGUCGg -3' miRNA: 3'- -UGUGCUAGUAGUUGCCGUCUCCCAGC- -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 17816 | 0.69 | 0.58353 |
Target: 5'- aACGCGggCAUCGAggacgacccgUGGCAGGucauuccgacagcauGGGUCGa -3' miRNA: 3'- -UGUGCuaGUAGUU----------GCCGUCU---------------CCCAGC- -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 19237 | 0.71 | 0.480857 |
Target: 5'- gGCACGccaccguguUCAUCGGCGGC-GAGGccGUCGa -3' miRNA: 3'- -UGUGCu--------AGUAGUUGCCGuCUCC--CAGC- -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 19332 | 0.67 | 0.703136 |
Target: 5'- gGCGCGGUCuUCGgacaggcgccagGCGGCGGAauugccguuGaGGUCGg -3' miRNA: 3'- -UGUGCUAGuAGU------------UGCCGUCU---------C-CCAGC- -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 26295 | 0.68 | 0.646897 |
Target: 5'- cGCGCGAUggaugcCAUCAACGGaCAGGcacGGUCu -3' miRNA: 3'- -UGUGCUA------GUAGUUGCC-GUCUc--CCAGc -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 30156 | 0.68 | 0.669517 |
Target: 5'- cGCGuCGGaCAUCAGCGGCAGuuGGcgccaGUCGg -3' miRNA: 3'- -UGU-GCUaGUAGUUGCCGUCu-CC-----CAGC- -5' |
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26744 | 3' | -53.2 | NC_005808.1 | + | 34550 | 0.66 | 0.778424 |
Target: 5'- gGCGCGcGUCAUgGACGGCGGcaAGGacggCGg -3' miRNA: 3'- -UGUGC-UAGUAgUUGCCGUC--UCCca--GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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