Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26745 | 3' | -56 | NC_005808.1 | + | 10234 | 0.73 | 0.209679 |
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Target: 5'- cGGCCGggGCcucgGCG-GCGACCGCAGCg- -3' miRNA: 3'- -CUGGCuaCG----UGCgCGUUGGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 9377 | 0.73 | 0.193009 |
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Target: 5'- cGGCCGcGUGCAuUGUGCAGCCACuGCa- -3' miRNA: 3'- -CUGGC-UACGU-GCGCGUUGGUGuCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 25161 | 0.74 | 0.182551 |
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Target: 5'- -cUCGAUGCugGCGgGGCCACuGCUc -3' miRNA: 3'- cuGGCUACGugCGCgUUGGUGuCGAa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 27005 | 0.75 | 0.145589 |
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Target: 5'- uGGCCGAUgGCGCgGUGCAuCCGCAGCg- -3' miRNA: 3'- -CUGGCUA-CGUG-CGCGUuGGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 41215 | 0.77 | 0.109032 |
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Target: 5'- aACCGuUGUGCGCGUGGCCGCGGCg- -3' miRNA: 3'- cUGGCuACGUGCGCGUUGGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 15418 | 1.06 | 0.000765 |
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Target: 5'- cGACCGAUGCACGCGCAACCACAGCUUg -3' miRNA: 3'- -CUGGCUACGUGCGCGUUGGUGUCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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