Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26745 | 3' | -56 | NC_005808.1 | + | 39758 | 0.7 | 0.328558 |
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Target: 5'- cGCUGGUGCugGCGCcgcugcgcguGGCCGCgAGCa- -3' miRNA: 3'- cUGGCUACGugCGCG----------UUGGUG-UCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 40249 | 0.67 | 0.459065 |
|
Target: 5'- aGGCCGAauugcggcGCACGCGC-GCCAC-GCa- -3' miRNA: 3'- -CUGGCUa-------CGUGCGCGuUGGUGuCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 41215 | 0.77 | 0.109032 |
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Target: 5'- aACCGuUGUGCGCGUGGCCGCGGCg- -3' miRNA: 3'- cUGGCuACGUGCGCGUUGGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 41244 | 0.69 | 0.36291 |
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Target: 5'- cGCCgGAUGCGCaaggcCGcCGACCACGGCUUc -3' miRNA: 3'- cUGG-CUACGUGc----GC-GUUGGUGUCGAA- -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 41488 | 0.72 | 0.253429 |
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Target: 5'- gGGCCGAUGCGCgacaugccGCGCGA-CACGGCc- -3' miRNA: 3'- -CUGGCUACGUG--------CGCGUUgGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 42427 | 0.72 | 0.246747 |
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Target: 5'- cGCCGGcauucUGCGCGCgGCAGCUugGGCa- -3' miRNA: 3'- cUGGCU-----ACGUGCG-CGUUGGugUCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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