Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26745 | 3' | -56 | NC_005808.1 | + | 4363 | 0.68 | 0.409232 |
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Target: 5'- cGGCCGGacgGCAgGCGgAugCGCAGCc- -3' miRNA: 3'- -CUGGCUa--CGUgCGCgUugGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 4205 | 0.68 | 0.399675 |
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Target: 5'- uGGCCGcgaGCACGuCGCGGCUuGCGGCUUg -3' miRNA: 3'- -CUGGCua-CGUGC-GCGUUGG-UGUCGAA- -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 3740 | 0.66 | 0.522836 |
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Target: 5'- cGCCGGUgguggGCACGuCGguGCCGCcGCUg -3' miRNA: 3'- cUGGCUA-----CGUGC-GCguUGGUGuCGAa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 939 | 0.68 | 0.447821 |
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Target: 5'- gGGCCGAucUGUccguccuGCGCGguGCCcacgGCAGCUUg -3' miRNA: 3'- -CUGGCU--ACG-------UGCGCguUGG----UGUCGAA- -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 819 | 0.68 | 0.438735 |
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Target: 5'- uGGCUGGgcuugaGCGCGCGCAGagACAGCg- -3' miRNA: 3'- -CUGGCUa-----CGUGCGCGUUggUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 391 | 0.66 | 0.522836 |
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Target: 5'- uGAgCGcgGCcaGCGCGCGACUguugaGCAGCa- -3' miRNA: 3'- -CUgGCuaCG--UGCGCGUUGG-----UGUCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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