Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26745 | 3' | -56 | NC_005808.1 | + | 4205 | 0.68 | 0.399675 |
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Target: 5'- uGGCCGcgaGCACGuCGCGGCUuGCGGCUUg -3' miRNA: 3'- -CUGGCua-CGUGC-GCGUUGG-UGUCGAA- -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 25298 | 0.68 | 0.399675 |
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Target: 5'- uGCCGGaaUGgAUGCGCGcCCGCGGCg- -3' miRNA: 3'- cUGGCU--ACgUGCGCGUuGGUGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 18880 | 0.68 | 0.399675 |
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Target: 5'- cACCGAgGCACuggccCGCGACCugGGCg- -3' miRNA: 3'- cUGGCUaCGUGc----GCGUUGGugUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 41244 | 0.69 | 0.36291 |
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Target: 5'- cGCCgGAUGCGCaaggcCGcCGACCACGGCUUc -3' miRNA: 3'- cUGG-CUACGUGc----GC-GUUGGUGUCGAA- -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 23514 | 0.69 | 0.36291 |
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Target: 5'- aGGCCGGccUGCAauCGCGUGACCuCGGCg- -3' miRNA: 3'- -CUGGCU--ACGU--GCGCGUUGGuGUCGaa -5' |
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| 26745 | 3' | -56 | NC_005808.1 | + | 27005 | 0.75 | 0.145589 |
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Target: 5'- uGGCCGAUgGCGCgGUGCAuCCGCAGCg- -3' miRNA: 3'- -CUGGCUA-CGUG-CGCGUuGGUGUCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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