Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26748 | 3' | -50.3 | NC_005808.1 | + | 20765 | 0.7 | 0.626502 |
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Target: 5'- ----uGCgCCGCGCUCGACUC-AUCGCg -3' miRNA: 3'- uuuauUG-GGCGUGGGUUGAGcUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 31178 | 0.7 | 0.626502 |
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Target: 5'- ---cGACCUGCACgCCGGCUCGc-CGCu -3' miRNA: 3'- uuuaUUGGGCGUG-GGUUGAGCuaGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 12745 | 0.71 | 0.614735 |
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Target: 5'- ---gGugCCGCGCCCAgacGCUUGG-CGCg -3' miRNA: 3'- uuuaUugGGCGUGGGU---UGAGCUaGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 1145 | 0.71 | 0.591264 |
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Target: 5'- --uUAGCUCGCGCUaCGGCggCGGUCACg -3' miRNA: 3'- uuuAUUGGGCGUGG-GUUGa-GCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 24509 | 0.72 | 0.556378 |
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Target: 5'- ---gGGCUgGCGCCCAcGC-CGAUCACg -3' miRNA: 3'- uuuaUUGGgCGUGGGU-UGaGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 18516 | 0.72 | 0.53345 |
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Target: 5'- ----uGCCCGUGCCCcGCgCGGUCGCc -3' miRNA: 3'- uuuauUGGGCGUGGGuUGaGCUAGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 14940 | 0.75 | 0.395234 |
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Target: 5'- ---cGACUgCGCGCCCAACUCGGcgaugcUCGCg -3' miRNA: 3'- uuuaUUGG-GCGUGGGUUGAGCU------AGUG- -5' |
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| 26748 | 3' | -50.3 | NC_005808.1 | + | 16982 | 1.07 | 0.002814 |
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Target: 5'- aAAAUAACCCGCACCCAACUCGAUCACc -3' miRNA: 3'- -UUUAUUGGGCGUGGGUUGAGCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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