Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26749 | 3' | -54.2 | NC_005808.1 | + | 40877 | 0.66 | 0.735939 |
Target: 5'- aGGAC-UUGGAC---GCCCUCgCCGGCa -3' miRNA: 3'- -CCUGcAACCUGuagUGGGGGaGGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 42290 | 0.66 | 0.703264 |
Target: 5'- cGGcACGcccUGGACGgaagagCGCgCCUUCCGGCg -3' miRNA: 3'- -CC-UGCa--ACCUGUa-----GUGgGGGAGGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 14401 | 0.66 | 0.692211 |
Target: 5'- aGGCGUagcgaagcUGGGCGUCGCCaggcgcgCCUgCGGCa -3' miRNA: 3'- cCUGCA--------ACCUGUAGUGGg------GGAgGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 22490 | 0.67 | 0.66658 |
Target: 5'- gGGGCGggcUGGGCuggucaugcccgccAUCAUCCCCg-CAGCg -3' miRNA: 3'- -CCUGCa--ACCUG--------------UAGUGGGGGagGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 8002 | 0.67 | 0.636273 |
Target: 5'- cGGCGUcGGACGU-GCCCUUggcgCCAGCu -3' miRNA: 3'- cCUGCAaCCUGUAgUGGGGGa---GGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 35406 | 0.67 | 0.62503 |
Target: 5'- --cCGaUGGcaagcccaGCGUCGCCCCCgaaUCCGACa -3' miRNA: 3'- ccuGCaACC--------UGUAGUGGGGG---AGGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 17922 | 0.68 | 0.591379 |
Target: 5'- uGGGCG-UGGACGUgGCCCgCggCgGGCg -3' miRNA: 3'- -CCUGCaACCUGUAgUGGGgGa-GgUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 18086 | 0.69 | 0.536124 |
Target: 5'- uGGACGUgaucggcgUGGGCGcCAgCCCCUaCGACu -3' miRNA: 3'- -CCUGCA--------ACCUGUaGUgGGGGAgGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 8935 | 0.7 | 0.493252 |
Target: 5'- cGGGCG-UGGACAguUCGCCgaaCUguUCCAGCa -3' miRNA: 3'- -CCUGCaACCUGU--AGUGGg--GG--AGGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 37505 | 0.7 | 0.462156 |
Target: 5'- cGGACGcUGGGCcggcCGCCUgUUCCAACc -3' miRNA: 3'- -CCUGCaACCUGua--GUGGGgGAGGUUG- -5' |
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26749 | 3' | -54.2 | NC_005808.1 | + | 17232 | 1.11 | 0.00068 |
Target: 5'- cGGACGUUGGACAUCACCCCCUCCAACc -3' miRNA: 3'- -CCUGCAACCUGUAGUGGGGGAGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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