Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26749 | 5' | -56.6 | NC_005808.1 | + | 16344 | 0.67 | 0.449906 |
Target: 5'- cAGGCCGGcGGGUGcccgUGCCuUCgGCGAc -3' miRNA: 3'- -UUCGGCC-CCUAUa---ACGGcAGgUGCUu -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 4821 | 0.67 | 0.439834 |
Target: 5'- gAGGUCGGGGccGgcGCCGUCguCGAAu -3' miRNA: 3'- -UUCGGCCCCuaUaaCGGCAGguGCUU- -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 39362 | 0.68 | 0.410411 |
Target: 5'- -cGCCGGGGGcuUAguaUGaCgGUCCGCGAc -3' miRNA: 3'- uuCGGCCCCU--AUa--AC-GgCAGGUGCUu -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 23936 | 0.68 | 0.391488 |
Target: 5'- cAGGCCGGGGGcgGgcGCCGcCCcCGAu -3' miRNA: 3'- -UUCGGCCCCUa-UaaCGGCaGGuGCUu -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 3922 | 0.72 | 0.211034 |
Target: 5'- --uUCGGGGAcgcgcUGCCGUCCGCGAu -3' miRNA: 3'- uucGGCCCCUaua--ACGGCAGGUGCUu -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 16485 | 0.73 | 0.199772 |
Target: 5'- cAGGUCGGGcgcGAUAUUGUCGUCCACc-- -3' miRNA: 3'- -UUCGGCCC---CUAUAACGGCAGGUGcuu -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 4536 | 0.73 | 0.189038 |
Target: 5'- -uGCUGGGcGGUcgUGCCG-CCGCGGAa -3' miRNA: 3'- uuCGGCCC-CUAuaACGGCaGGUGCUU- -5' |
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26749 | 5' | -56.6 | NC_005808.1 | + | 17266 | 1.04 | 0.000981 |
Target: 5'- cAAGCCGGGGAUAUUGCCGUCCACGAAg -3' miRNA: 3'- -UUCGGCCCCUAUAACGGCAGGUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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