Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2675 | 5' | -58.6 | NC_001491.2 | + | 48461 | 0.73 | 0.377746 |
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Target: 5'- aGCUGcGUGGACGAGCgugaccUGGCCGguaaacuguaugugGCCa- -3' miRNA: 3'- aCGAC-CACCUGCUCG------ACCGGU--------------UGGag -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 38573 | 0.73 | 0.372045 |
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Target: 5'- -aCUGGUGGACuGGCUGGUCGAaaggCUCg -3' miRNA: 3'- acGACCACCUGcUCGACCGGUUg---GAG- -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 48525 | 0.74 | 0.325622 |
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Target: 5'- gGUUGGaggccgUGGugGAGCUGGCCAggugcACCg- -3' miRNA: 3'- aCGACC------ACCugCUCGACCGGU-----UGGag -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 28754 | 0.76 | 0.246061 |
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Target: 5'- gGCUGGUGGACGGcgucccccGCUGGgCCAugCg- -3' miRNA: 3'- aCGACCACCUGCU--------CGACC-GGUugGag -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 67320 | 1.1 | 0.00123 |
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Target: 5'- cUGCUGGUGGACGAGCUGGCCAACCUCa -3' miRNA: 3'- -ACGACCACCUGCUCGACCGGUUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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