Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2675 | 5' | -58.6 | NC_001491.2 | + | 17108 | 0.66 | 0.762712 |
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Target: 5'- aGUUGGUGGugGGuaagcguuguccGUcgagaaagagcaagUGGCCAACCa- -3' miRNA: 3'- aCGACCACCugCU------------CG--------------ACCGGUUGGag -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 5327 | 0.66 | 0.765547 |
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Target: 5'- gGCUGGgugccggGGACGccGGCUGGgCGGCa-- -3' miRNA: 3'- aCGACCa------CCUGC--UCGACCgGUUGgag -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 110721 | 0.66 | 0.765547 |
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Target: 5'- cGCUGucgcGcCGAGUUGGCCAGCgUCu -3' miRNA: 3'- aCGACcac-CuGCUCGACCGGUUGgAG- -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 33882 | 0.66 | 0.773986 |
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Target: 5'- cGCUGGUGcGCuacaacgccgaggGGGCccgGGCCcgGGCCUCg -3' miRNA: 3'- aCGACCACcUG-------------CUCGa--CCGG--UUGGAG- -5' |
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| 2675 | 5' | -58.6 | NC_001491.2 | + | 1811 | 0.66 | 0.784161 |
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Target: 5'- gUGUaGGccacguUGGAgGAGC-GGCaCAGCCUCa -3' miRNA: 3'- -ACGaCC------ACCUgCUCGaCCG-GUUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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