Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26750 | 3' | -52.9 | NC_005808.1 | + | 32458 | 0.66 | 0.721618 |
Target: 5'- cGCCUUccagAAGAAagGAGGCGuGGccGGUACGAGg -3' miRNA: 3'- -CGGAG----UUCUU--UUUCGC-CC--CCGUGCUU- -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 13460 | 0.67 | 0.699072 |
Target: 5'- gGCCUCGGGc----GCGGugucGGGCAUGAc -3' miRNA: 3'- -CGGAGUUCuuuuuCGCC----CCCGUGCUu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 15346 | 0.67 | 0.687686 |
Target: 5'- uGCCUCGGccAAGAGCcuGGGGCGCc-- -3' miRNA: 3'- -CGGAGUUcuUUUUCGc-CCCCGUGcuu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 6876 | 0.68 | 0.618566 |
Target: 5'- gGCCUCGaacagaacGGGGAAAGCGaGGcGCACGu- -3' miRNA: 3'- -CGGAGU--------UCUUUUUCGCcCC-CGUGCuu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 8983 | 0.68 | 0.59896 |
Target: 5'- cGCCUCGAuuuccucgccgcGCGGGGGCGCu-- -3' miRNA: 3'- -CGGAGUUcuuuuu------CGCCCCCGUGcuu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 38345 | 0.68 | 0.595508 |
Target: 5'- cGCCUCAAGAccuacGGCGGGaagcuGGCA-GAAa -3' miRNA: 3'- -CGGAGUUCUuuu--UCGCCC-----CCGUgCUU- -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 22039 | 0.69 | 0.572586 |
Target: 5'- uGgCUCAuGAGcagcAAGCGGGGGCGCc-- -3' miRNA: 3'- -CgGAGUuCUUu---UUCGCCCCCGUGcuu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 23570 | 0.69 | 0.561201 |
Target: 5'- cGCCggCAGGugcGGGCgGGGGGCgACGAu -3' miRNA: 3'- -CGGa-GUUCuuuUUCG-CCCCCG-UGCUu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 632 | 0.7 | 0.516373 |
Target: 5'- aGCCUCGgcgcgggugaacGGGucAGGCGGGGGCuuuUGGu -3' miRNA: 3'- -CGGAGU------------UCUuuUUCGCCCCCGu--GCUu -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 10462 | 0.75 | 0.274554 |
Target: 5'- gGCCUUGAGGGAuu-CGGGGGCGuCGAGg -3' miRNA: 3'- -CGGAGUUCUUUuucGCCCCCGU-GCUU- -5' |
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26750 | 3' | -52.9 | NC_005808.1 | + | 17922 | 1.09 | 0.001055 |
Target: 5'- cGCCUCAAGAAAAAGCGGGGGCACGAAg -3' miRNA: 3'- -CGGAGUUCUUUUUCGCCCCCGUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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