Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26753 | 3' | -60.5 | NC_005808.1 | + | 30391 | 0.68 | 0.277745 |
Target: 5'- gGGUCGGUGAuaaCGGCGUCCaCGCUu -3' miRNA: 3'- -CCAGCUACUccaGCCGCGGGcGCGAc -5' |
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26753 | 3' | -60.5 | NC_005808.1 | + | 35167 | 0.67 | 0.337029 |
Target: 5'- cGUCGAggaAGuGUCcgGGCGCCCGCuGCUc -3' miRNA: 3'- cCAGCUac-UC-CAG--CCGCGGGCG-CGAc -5' |
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26753 | 3' | -60.5 | NC_005808.1 | + | 35211 | 0.7 | 0.221325 |
Target: 5'- uGGUgGA--AGGUcgccgCGGUGCCCGcCGCUGg -3' miRNA: 3'- -CCAgCUacUCCA-----GCCGCGGGC-GCGAC- -5' |
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26753 | 3' | -60.5 | NC_005808.1 | + | 40565 | 0.68 | 0.298949 |
Target: 5'- cGUCGccGAGG-CGGCcgguauGCCgGUGCUGg -3' miRNA: 3'- cCAGCuaCUCCaGCCG------CGGgCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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