Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26754 | 3' | -61.2 | NC_005808.1 | + | 31080 | 0.66 | 0.325457 |
Target: 5'- gGGCCU-GGUgccgcugcucaccUCGGuGCAGGCCGgCGAg -3' miRNA: 3'- -UUGGGuCCAa------------AGCC-CGUCCGGCgGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 9928 | 0.66 | 0.32076 |
Target: 5'- uGCUCGGGcuucacgUCGGGCAGcuucgcGgCGCCGAa -3' miRNA: 3'- uUGGGUCCaa-----AGCCCGUC------CgGCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 5820 | 0.66 | 0.32076 |
Target: 5'- cGCCCugguuggucaGGGUUUCGGGCuGGGUauuagggacuuCGCUGGu -3' miRNA: 3'- uUGGG----------UCCAAAGCCCG-UCCG-----------GCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 3785 | 0.66 | 0.298041 |
Target: 5'- cGACCCAcacGGUacggUCGGGCagcaugaccAGGCgGUCGGg -3' miRNA: 3'- -UUGGGU---CCAa---AGCCCG---------UCCGgCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 2510 | 0.67 | 0.2836 |
Target: 5'- cAACCCG---UUCGGGCuGGGCgUGCCGGu -3' miRNA: 3'- -UUGGGUccaAAGCCCG-UCCG-GCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 4538 | 0.67 | 0.27659 |
Target: 5'- aGACCacgaAGGUcUUGGGCuugAGGCCgaagucGCCGAg -3' miRNA: 3'- -UUGGg---UCCAaAGCCCG---UCCGG------CGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 18065 | 0.67 | 0.262985 |
Target: 5'- cGGCgCGGGgugCGuGCuGGGCCGCCGAa -3' miRNA: 3'- -UUGgGUCCaaaGCcCG-UCCGGCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 5536 | 0.68 | 0.243604 |
Target: 5'- cGCCCAGGUUcCGccCAGGUCGCCc- -3' miRNA: 3'- uUGGGUCCAAaGCccGUCCGGCGGcu -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 23715 | 0.68 | 0.237413 |
Target: 5'- cGCCCAGGUcgCGGGCcAGuGCC-UCGGu -3' miRNA: 3'- uUGGGUCCAaaGCCCG-UC-CGGcGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 8691 | 0.68 | 0.237413 |
Target: 5'- cGCCCAGGUgugCGccgcGCAGauuGCUGCCGAa -3' miRNA: 3'- uUGGGUCCAaa-GCc---CGUC---CGGCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 39088 | 0.68 | 0.233762 |
Target: 5'- gAACUCGGGUUcUCGcGcugcguacugguacaGCGGGCCGUCGAa -3' miRNA: 3'- -UUGGGUCCAA-AGC-C---------------CGUCCGGCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 13327 | 0.68 | 0.225426 |
Target: 5'- cGGCgUAGGcUUUC-GGCAGGUCGCCGc -3' miRNA: 3'- -UUGgGUCC-AAAGcCCGUCCGGCGGCu -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 8539 | 0.69 | 0.202991 |
Target: 5'- uGCCCAGGUaUC--GCAGGCCGCgcuCGAc -3' miRNA: 3'- uUGGGUCCAaAGccCGUCCGGCG---GCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 31229 | 0.69 | 0.202991 |
Target: 5'- cACCgAGGUgcCGGGCgagcAGGUCGCCa- -3' miRNA: 3'- uUGGgUCCAaaGCCCG----UCCGGCGGcu -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 32351 | 0.69 | 0.197693 |
Target: 5'- cGCCCAGG--UCGcuGCGGucGCCGCCGAg -3' miRNA: 3'- uUGGGUCCaaAGCc-CGUC--CGGCGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 718 | 0.69 | 0.18746 |
Target: 5'- cGCCCAGGaUagGGGC-GGCUuuGCCGAc -3' miRNA: 3'- uUGGGUCCaAagCCCGuCCGG--CGGCU- -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 27746 | 0.7 | 0.182519 |
Target: 5'- cGCCCAGcGUg-CGGcGCuGGCCGCCa- -3' miRNA: 3'- uUGGGUC-CAaaGCC-CGuCCGGCGGcu -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 2671 | 0.7 | 0.177695 |
Target: 5'- cGCCCAGGUgUUCGaucagccacGGCAGGuuGUCGu -3' miRNA: 3'- uUGGGUCCA-AAGC---------CCGUCCggCGGCu -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 31672 | 0.7 | 0.16884 |
Target: 5'- uGCCCgAGGcccugcaaaccggCGGGCAGGUCGCCa- -3' miRNA: 3'- uUGGG-UCCaaa----------GCCCGUCCGGCGGcu -5' |
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26754 | 3' | -61.2 | NC_005808.1 | + | 7678 | 0.7 | 0.163896 |
Target: 5'- gGGgCCAGGUcgUCGGGCuugaccucGGCgGCCGGc -3' miRNA: 3'- -UUgGGUCCAa-AGCCCGu-------CCGgCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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