Results 61 - 65 of 65 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26755 | 3' | -54.4 | NC_005808.1 | + | 24982 | 0.79 | 0.130361 |
Target: 5'- aCGCGCCCacccaccagcacaaAGGggGCCGACGAcAGCGGg -3' miRNA: 3'- gGCGCGGG--------------UCCaaCGGUUGCU-UUGCU- -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 29444 | 0.79 | 0.127437 |
Target: 5'- gUCGCGCCCAGG-UGCUccuggcaccaggcGACGAAGCGc -3' miRNA: 3'- -GGCGCGGGUCCaACGG-------------UUGCUUUGCu -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 7498 | 0.82 | 0.0783 |
Target: 5'- aCCGCGCCCagcAGGUUGCCGAgGAuGGCGu -3' miRNA: 3'- -GGCGCGGG---UCCAACGGUUgCU-UUGCu -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 69 | 0.88 | 0.032213 |
Target: 5'- uCCGCGCCUAgGGUUGCCu-CGAAGCGAg -3' miRNA: 3'- -GGCGCGGGU-CCAACGGuuGCUUUGCU- -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 21343 | 1.11 | 0.000688 |
Target: 5'- aCCGCGCCCAGGUUGCCAACGAAACGAu -3' miRNA: 3'- -GGCGCGGGUCCAACGGUUGCUUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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