miRNA: 3'- ggCGCGGGucCAaCGGUUGCUuu-GCU- -5'
miRNA: 3'- gGCGCGG----GUCCAaCG-GUUGCUuUGCU- -5'
miRNA: 3'- -GGCGCGGGuccaaCGGu-UGCUUUGCU- -5'
miRNA: 3'- -GGCGCGGGUccaaCGGUUg-CUUUGCU- -5'
miRNA: 3'- -GGCGCGGgU-CCaaCgGUUGCUu----UGCU- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26755 | 3' | -54.4 | NC_005808.1 | + | 25548 | 0.66 | 0.716076 |
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Target: 5'- -aGCGCCCccGUaGCCGAUGAuguCGGc -3' miRNA: 3'- ggCGCGGGucCAaCGGUUGCUuu-GCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 33066 | 0.66 | 0.716076 |
|
Target: 5'- gUGCGCUucauCGGGUcGCgCAGCGAcGCGGc -3' miRNA: 3'- gGCGCGG----GUCCAaCG-GUUGCUuUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 7777 | 0.66 | 0.726902 |
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Target: 5'- cUCGCGCCUuc---GCCcuGCGAGGCGAu -3' miRNA: 3'- -GGCGCGGGuccaaCGGu-UGCUUUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 30505 | 0.66 | 0.726902 |
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Target: 5'- aCCGUGCUCAaccaGCCGAagGAAGCGAa -3' miRNA: 3'- -GGCGCGGGUccaaCGGUUg-CUUUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 8555 | 0.66 | 0.726902 |
|
Target: 5'- gCCGCGCCgAuGGcgGgCAGCGAcaggcGCGAc -3' miRNA: 3'- -GGCGCGGgU-CCaaCgGUUGCUu----UGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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