Results 61 - 65 of 65 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26755 | 3' | -54.4 | NC_005808.1 | + | 39877 | 0.67 | 0.649729 |
Target: 5'- gCCGCGCUCaAGcGUccgGCCAACGuguucaccACGAa -3' miRNA: 3'- -GGCGCGGG-UC-CAa--CGGUUGCuu------UGCU- -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 39952 | 0.66 | 0.694177 |
Target: 5'- aCCGCugGCCCuuccgcaaaguGGUgGCUGACGAaucGACGAa -3' miRNA: 3'- -GGCG--CGGGu----------CCAaCGGUUGCU---UUGCU- -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 41528 | 0.67 | 0.660894 |
Target: 5'- aCGCGCgCCGGGUcGCaCAggaaauccGCG-AGCGAa -3' miRNA: 3'- gGCGCG-GGUCCAaCG-GU--------UGCuUUGCU- -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 41977 | 0.72 | 0.341274 |
Target: 5'- cCCGCGCCgAGGcugagGCCAucaucgccgacaugGCGAAGCa- -3' miRNA: 3'- -GGCGCGGgUCCaa---CGGU--------------UGCUUUGcu -5' |
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26755 | 3' | -54.4 | NC_005808.1 | + | 42068 | 0.67 | 0.672031 |
Target: 5'- cCCGCGCacguCCgAGGUauucgGCCAGCGAuggcccAACGu -3' miRNA: 3'- -GGCGCG----GG-UCCAa----CGGUUGCU------UUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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