Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26755 | 3' | -54.4 | NC_005808.1 | + | 6639 | 0.71 | 0.396859 |
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Target: 5'- gCGCGCggacacguaCAGGUUGCCGGgGAAGCc- -3' miRNA: 3'- gGCGCGg--------GUCCAACGGUUgCUUUGcu -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 3925 | 0.7 | 0.444918 |
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Target: 5'- gCCGCGCCCccaugcGaGUUugaaaGCCAGCGAAcgGCGGa -3' miRNA: 3'- -GGCGCGGGu-----C-CAA-----CGGUUGCUU--UGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 36988 | 0.7 | 0.485615 |
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Target: 5'- aUCGaCGCCguGGcuaCCAACGAGGCGGa -3' miRNA: 3'- -GGC-GCGGguCCaacGGUUGCUUUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 27744 | 0.68 | 0.55847 |
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Target: 5'- -gGCGCCCAGcGUgcggcgcuggccGCCAACGGcaucgaccugGGCGAa -3' miRNA: 3'- ggCGCGGGUC-CAa-----------CGGUUGCU----------UUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 69 | 0.88 | 0.032213 |
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Target: 5'- uCCGCGCCUAgGGUUGCCu-CGAAGCGAg -3' miRNA: 3'- -GGCGCGGGU-CCAACGGuuGCUUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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