Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26755 | 3' | -54.4 | NC_005808.1 | + | 16000 | 0.69 | 0.528 |
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Target: 5'- gCCGCGCgCAGGgacaggucGCCGgugACGAAACc- -3' miRNA: 3'- -GGCGCGgGUCCaa------CGGU---UGCUUUGcu -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 15154 | 0.69 | 0.528 |
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Target: 5'- aUGCGCCCAGGgcgacgGCUGAUGuuGCu- -3' miRNA: 3'- gGCGCGGGUCCaa----CGGUUGCuuUGcu -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 19728 | 0.69 | 0.506619 |
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Target: 5'- gCCGCgcaucGCCCugcuugggcuGGUUGCCGuuguugccggccACGAGGCGGu -3' miRNA: 3'- -GGCG-----CGGGu---------CCAACGGU------------UGCUUUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 32417 | 0.7 | 0.496067 |
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Target: 5'- gCCGCGCgCCAcGUcUGCCAagACGAcggcAGCGAc -3' miRNA: 3'- -GGCGCG-GGUcCA-ACGGU--UGCU----UUGCU- -5' |
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| 26755 | 3' | -54.4 | NC_005808.1 | + | 21343 | 1.11 | 0.000688 |
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Target: 5'- aCCGCGCCCAGGUUGCCAACGAAACGAu -3' miRNA: 3'- -GGCGCGGGUCCAACGGUUGCUUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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