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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26756 | 3' | -58.2 | NC_005808.1 | + | 8560 | 0.68 | 0.399289 |
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Target: 5'- gCCgAugGCGGGCagcgACAG-GCGCGacACg -3' miRNA: 3'- -GGgUugCGCCCG----UGUCaCGCGCcuUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 7378 | 0.66 | 0.476356 |
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Target: 5'- gUCCAGCgGCGGGCAC----CGCGGcGACc -3' miRNA: 3'- -GGGUUG-CGCCCGUGucacGCGCC-UUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 6619 | 0.73 | 0.188064 |
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Target: 5'- aCCAgcggGCGCGuGGCGCuGcGCGCGGAcACg -3' miRNA: 3'- gGGU----UGCGC-CCGUGuCaCGCGCCU-UG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 5861 | 0.66 | 0.507117 |
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Target: 5'- gCUAuUGUGGGCGCAGaGCGCGa--- -3' miRNA: 3'- gGGUuGCGCCCGUGUCaCGCGCcuug -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 5747 | 0.72 | 0.21481 |
|
Target: 5'- gCCCAGCGCgccGGGCAgCgAGUGCGCcaGGGCc -3' miRNA: 3'- -GGGUUGCG---CCCGU-G-UCACGCGc-CUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 4037 | 0.7 | 0.278084 |
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Target: 5'- gCC-GCGCGGGUcgagcGCAGccGgGCGGAACu -3' miRNA: 3'- gGGuUGCGCCCG-----UGUCa-CgCGCCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 3741 | 0.67 | 0.427266 |
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Target: 5'- gCCgGugGUGGGCACgucGGUGCcgccGCuGGACg -3' miRNA: 3'- -GGgUugCGCCCGUG---UCACG----CGcCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 1877 | 0.66 | 0.507117 |
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Target: 5'- gCCCGugGCcGGCGCuggccgGGUGgGC-GAACa -3' miRNA: 3'- -GGGUugCGcCCGUG------UCACgCGcCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 634 | 0.71 | 0.23238 |
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Target: 5'- cCUCGGCGCGGGUgaACGGgucagGCG-GGGGCu -3' miRNA: 3'- -GGGUUGCGCCCG--UGUCa----CGCgCCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 462 | 0.69 | 0.346982 |
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Target: 5'- gCCAGCuuGCGGGCGCuGU-CGCGGu-- -3' miRNA: 3'- gGGUUG--CGCCCGUGuCAcGCGCCuug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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