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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26756 | 3' | -58.2 | NC_005808.1 | + | 6619 | 0.73 | 0.188064 |
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Target: 5'- aCCAgcggGCGCGuGGCGCuGcGCGCGGAcACg -3' miRNA: 3'- gGGU----UGCGC-CCGUGuCaCGCGCCU-UG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 5747 | 0.72 | 0.21481 |
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Target: 5'- gCCCAGCGCgccGGGCAgCgAGUGCGCcaGGGCc -3' miRNA: 3'- -GGGUUGCG---CCCGU-G-UCACGCGc-CUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 30447 | 0.72 | 0.220537 |
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Target: 5'- cCCCGacgccuACGCGGaaGCGCAGcGCGUGGcGCg -3' miRNA: 3'- -GGGU------UGCGCC--CGUGUCaCGCGCCuUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 25190 | 0.71 | 0.224623 |
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Target: 5'- -gCGACGUGGGCgccgcugaaucgaaGCGGUGCGuCGGcGCg -3' miRNA: 3'- ggGUUGCGCCCG--------------UGUCACGC-GCCuUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 634 | 0.71 | 0.23238 |
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Target: 5'- cCUCGGCGCGGGUgaACGGgucagGCG-GGGGCu -3' miRNA: 3'- -GGGUUGCGCCCG--UGUCa----CGCgCCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 14778 | 0.71 | 0.251144 |
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Target: 5'- gCCAcCGCGGGCAgcGcGCGCGaGGACg -3' miRNA: 3'- gGGUuGCGCCCGUguCaCGCGC-CUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 15162 | 0.71 | 0.251144 |
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Target: 5'- gCCAGggccugcggcCGcCGGGCGCGGUGCGCcaGGcGCg -3' miRNA: 3'- gGGUU----------GC-GCCCGUGUCACGCG--CCuUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 38383 | 0.71 | 0.251144 |
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Target: 5'- -gCGACGauGGCACGGUGCGCGc--- -3' miRNA: 3'- ggGUUGCgcCCGUGUCACGCGCcuug -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 32586 | 0.7 | 0.299764 |
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Target: 5'- aCCAGgGCGGGgGCcgGGcGCGCGGcACu -3' miRNA: 3'- gGGUUgCGCCCgUG--UCaCGCGCCuUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 37816 | 0.79 | 0.072802 |
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Target: 5'- gCCGGCGUgcuGGGCGCAGUGUGgGcGAACg -3' miRNA: 3'- gGGUUGCG---CCCGUGUCACGCgC-CUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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