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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26756 | 3' | -58.2 | NC_005808.1 | + | 40834 | 0.69 | 0.338755 |
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Target: 5'- gCCCGACGCGccaGGCGCAG-GC-CGGccauGACc -3' miRNA: 3'- -GGGUUGCGC---CCGUGUCaCGcGCC----UUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 25084 | 0.66 | 0.496765 |
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Target: 5'- aCCCAGCGCaGcGCGCccgGCGCcguGGGAUa -3' miRNA: 3'- -GGGUUGCGcC-CGUGucaCGCG---CCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 24063 | 0.66 | 0.476356 |
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Target: 5'- uUCC-AUGCcGcGCACAGUagcggcgaccGCGCGGGGCa -3' miRNA: 3'- -GGGuUGCGcC-CGUGUCA----------CGCGCCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 36161 | 0.67 | 0.456371 |
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Target: 5'- gCCuuCGCGGGCGguG-GUGuCGcGAGCg -3' miRNA: 3'- gGGuuGCGCCCGUguCaCGC-GC-CUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 3741 | 0.67 | 0.427266 |
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Target: 5'- gCCgGugGUGGGCACgucGGUGCcgccGCuGGACg -3' miRNA: 3'- -GGgUugCGCCCGUG---UCACG----CGcCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 8841 | 0.67 | 0.417811 |
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Target: 5'- gCCgCAACGCGGGC-CAGcGCGuUGGc-- -3' miRNA: 3'- -GG-GUUGCGCCCGuGUCaCGC-GCCuug -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 31033 | 0.67 | 0.407559 |
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Target: 5'- gCCGaggcaaucGCGCGGGC-CAacGUGCGCGaaggcccGAACa -3' miRNA: 3'- gGGU--------UGCGCCCGuGU--CACGCGC-------CUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 29617 | 0.68 | 0.390227 |
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Target: 5'- gUCCAGCGcCGGGCGCuuGUG-GCcGGGCu -3' miRNA: 3'- -GGGUUGC-GCCCGUGu-CACgCGcCUUG- -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 462 | 0.69 | 0.346982 |
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Target: 5'- gCCAGCuuGCGGGCGCuGU-CGCGGu-- -3' miRNA: 3'- gGGUUG--CGCCCGUGuCAcGCGCCuug -5' |
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| 26756 | 3' | -58.2 | NC_005808.1 | + | 21949 | 1.01 | 0.001661 |
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Target: 5'- gCCCAACG-GGGCACAGUGCGCGGAACg -3' miRNA: 3'- -GGGUUGCgCCCGUGUCACGCGCCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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