Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26757 | 5' | -57.4 | NC_005808.1 | + | 309 | 0.7 | 0.335617 |
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Target: 5'- cGCucUUCCGUccaGGGCGUGCCguaCCGGGg -3' miRNA: 3'- -CGucAAGGCG---CUCGCGCGGaa-GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 42284 | 0.71 | 0.29982 |
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Target: 5'- cCGGUaCgGCacgcccuggacggaaGAGCGCGCCUUCCGGc -3' miRNA: 3'- cGUCAaGgCG---------------CUCGCGCGGAAGGUCc -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 8302 | 0.71 | 0.275308 |
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Target: 5'- gGCAGUgcagCCaGCGGuGCGCGCUgcUCGGGg -3' miRNA: 3'- -CGUCAa---GG-CGCU-CGCGCGGaaGGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 10554 | 0.74 | 0.166888 |
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Target: 5'- aUAGUUgUGCGAGCGCGCCggguucaaugUCguGGg -3' miRNA: 3'- cGUCAAgGCGCUCGCGCGGa---------AGguCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 9221 | 0.75 | 0.141559 |
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Target: 5'- cGCAcGUUCUGCG-GCGCGCCcggcggcagUUCCAGu -3' miRNA: 3'- -CGU-CAAGGCGCuCGCGCGG---------AAGGUCc -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 17294 | 0.76 | 0.123193 |
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Target: 5'- cCGGcgCCGCGGGCGCGCauccaUUCCGGc -3' miRNA: 3'- cGUCaaGGCGCUCGCGCGg----AAGGUCc -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 36138 | 0.79 | 0.07393 |
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Target: 5'- uGCAGUUCCuGCGcgacGGCGaCGCCUUCgCGGGc -3' miRNA: 3'- -CGUCAAGG-CGC----UCGC-GCGGAAG-GUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 28223 | 0.8 | 0.065888 |
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Target: 5'- --cGUUCCGCG-GCGCggaugcccccGCCUUCCAGGa -3' miRNA: 3'- cguCAAGGCGCuCGCG----------CGGAAGGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 23511 | 1.11 | 0.000345 |
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Target: 5'- gGCAGUUCCGCGAGCGCGCCUUCCAGGc -3' miRNA: 3'- -CGUCAAGGCGCUCGCGCGGAAGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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