Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26757 | 5' | -57.4 | NC_005808.1 | + | 15509 | 0.67 | 0.476713 |
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Target: 5'- uGCAGUUCUGCGgugaugGGCaggccgauaugcacgGUGCCggccuccacgUCCAGGu -3' miRNA: 3'- -CGUCAAGGCGC------UCG---------------CGCGGa---------AGGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 15171 | 0.67 | 0.493037 |
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Target: 5'- uGCGG--CCGcCGGGCGCGgUgcgCCAGGc -3' miRNA: 3'- -CGUCaaGGC-GCUCGCGCgGaa-GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 15084 | 0.67 | 0.494066 |
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Target: 5'- uGCAGgcugcuuugCUGCGAccGCGCGCCguaauagcugccuaCCAGGc -3' miRNA: 3'- -CGUCaa-------GGCGCU--CGCGCGGaa------------GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 14812 | 0.66 | 0.545567 |
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Target: 5'- uGCAccUCgGCG-GCGCuGCCUUcgcCCAGGu -3' miRNA: 3'- -CGUcaAGgCGCuCGCG-CGGAA---GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 13125 | 0.66 | 0.556301 |
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Target: 5'- cGCGGaacuggCCGCGcGCuucguCGCCUggugCCAGGa -3' miRNA: 3'- -CGUCaa----GGCGCuCGc----GCGGAa---GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 10554 | 0.74 | 0.166888 |
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Target: 5'- aUAGUUgUGCGAGCGCGCCggguucaaugUCguGGg -3' miRNA: 3'- cGUCAAgGCGCUCGCGCGGa---------AGguCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 9221 | 0.75 | 0.141559 |
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Target: 5'- cGCAcGUUCUGCG-GCGCGCCcggcggcagUUCCAGu -3' miRNA: 3'- -CGU-CAAGGCGCuCGCGCGG---------AAGGUCc -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 8302 | 0.71 | 0.275308 |
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Target: 5'- gGCAGUgcagCCaGCGGuGCGCGCUgcUCGGGg -3' miRNA: 3'- -CGUCAa---GG-CGCU-CGCGCGGaaGGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 309 | 0.7 | 0.335617 |
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Target: 5'- cGCucUUCCGUccaGGGCGUGCCguaCCGGGg -3' miRNA: 3'- -CGucAAGGCG---CUCGCGCGGaa-GGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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