Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26757 | 5' | -57.4 | NC_005808.1 | + | 42284 | 0.71 | 0.29982 |
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Target: 5'- cCGGUaCgGCacgcccuggacggaaGAGCGCGCCUUCCGGc -3' miRNA: 3'- cGUCAaGgCG---------------CUCGCGCGGAAGGUCc -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 26233 | 0.7 | 0.3438 |
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Target: 5'- cGCAGUUuuGCGcGCGuCGCCgaaggCaCGGGc -3' miRNA: 3'- -CGUCAAggCGCuCGC-GCGGaa---G-GUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 15509 | 0.67 | 0.476713 |
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Target: 5'- uGCAGUUCUGCGgugaugGGCaggccgauaugcacgGUGCCggccuccacgUCCAGGu -3' miRNA: 3'- -CGUCAAGGCGC------UCG---------------CGCGGa---------AGGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 15084 | 0.67 | 0.494066 |
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Target: 5'- uGCAGgcugcuuugCUGCGAccGCGCGCCguaauagcugccuaCCAGGc -3' miRNA: 3'- -CGUCaa-------GGCGCU--CGCGCGGaa------------GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 28642 | 0.66 | 0.545567 |
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Target: 5'- cCAGUUCCGCGcGCaGUGCCagaucggugCCGGcGg -3' miRNA: 3'- cGUCAAGGCGCuCG-CGCGGaa-------GGUC-C- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 34423 | 0.66 | 0.545567 |
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Target: 5'- gGCAGga-CGCca--GCGCCUUCCuGGg -3' miRNA: 3'- -CGUCaagGCGcucgCGCGGAAGGuCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 14812 | 0.66 | 0.545567 |
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Target: 5'- uGCAccUCgGCG-GCGCuGCCUUcgcCCAGGu -3' miRNA: 3'- -CGUcaAGgCGCuCGCG-CGGAA---GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 17438 | 0.66 | 0.567097 |
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Target: 5'- aGCAGUggccCCGCcAGCaucgaggccagGUGCCacgCCAGGg -3' miRNA: 3'- -CGUCAa---GGCGcUCG-----------CGCGGaa-GGUCC- -5' |
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| 26757 | 5' | -57.4 | NC_005808.1 | + | 23511 | 1.11 | 0.000345 |
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Target: 5'- gGCAGUUCCGCGAGCGCGCCUUCCAGGc -3' miRNA: 3'- -CGUCAAGGCGCUCGCGCGGAAGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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