Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26758 | 3' | -54.2 | NC_005808.1 | + | 32852 | 0.71 | 0.440172 |
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Target: 5'- -gAGGCGAAgcgcGCCCACCacgagcAGUUCgCcCGCg -3' miRNA: 3'- cgUCCGCUU----UGGGUGG------UCAAG-GaGCG- -5' |
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| 26758 | 3' | -54.2 | NC_005808.1 | + | 31453 | 0.69 | 0.522258 |
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Target: 5'- cGCAGGCG--GCCCAgCAGcaggCCgaacugcUCGCa -3' miRNA: 3'- -CGUCCGCuuUGGGUgGUCaa--GG-------AGCG- -5' |
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| 26758 | 3' | -54.2 | NC_005808.1 | + | 19111 | 0.69 | 0.545104 |
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Target: 5'- cGCAGGuCGAGauucccacgGCgCACCuGUUCCaCGCc -3' miRNA: 3'- -CGUCC-GCUU---------UGgGUGGuCAAGGaGCG- -5' |
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| 26758 | 3' | -54.2 | NC_005808.1 | + | 29619 | 0.68 | 0.567155 |
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Target: 5'- gGCGGGCGGuuuucggaaCUGCCGGUgUCCauUCGCg -3' miRNA: 3'- -CGUCCGCUuug------GGUGGUCA-AGG--AGCG- -5' |
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| 26758 | 3' | -54.2 | NC_005808.1 | + | 7252 | 0.68 | 0.567155 |
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Target: 5'- cGCGGGCcGAugUCGCCGG--CCUUGUg -3' miRNA: 3'- -CGUCCGcUUugGGUGGUCaaGGAGCG- -5' |
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| 26758 | 3' | -54.2 | NC_005808.1 | + | 4372 | 0.78 | 0.165942 |
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Target: 5'- gGCAGGCGGAugCgcaGCCAGgcgCCgUCGCg -3' miRNA: 3'- -CGUCCGCUUugGg--UGGUCaa-GG-AGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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