Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26758 | 5' | -59.5 | NC_005808.1 | + | 42121 | 0.67 | 0.353312 |
Target: 5'- cUGCCGAGGcauccgaccGCGacaGCGCCCGCa -3' miRNA: 3'- uGCGGUUCCuuca-----CGCgg-UGCGGGCG- -5' |
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26758 | 5' | -59.5 | NC_005808.1 | + | 42375 | 0.78 | 0.059826 |
Target: 5'- cGCGCCAAugccGAGGUGCGCCAguucgccguguCGCCCGg -3' miRNA: 3'- -UGCGGUUc---CUUCACGCGGU-----------GCGGGCg -5' |
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26758 | 5' | -59.5 | NC_005808.1 | + | 42450 | 0.76 | 0.079682 |
Target: 5'- cCGCCugcgGGGAAGccugGCGCCACGCCguaGCu -3' miRNA: 3'- uGCGGu---UCCUUCa---CGCGGUGCGGg--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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