Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26762 | 5' | -54.9 | NC_005808.1 | + | 26425 | 1.11 | 0.000536 |
Target: 5'- aCUCAUGCCCUCGACCUCGUGCAGUUCg -3' miRNA: 3'- -GAGUACGGGAGCUGGAGCACGUCAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 21572 | 0.75 | 0.198837 |
Target: 5'- cCUCGUGCCgCUCGGCCacuUCGgugGCGGUcaUCu -3' miRNA: 3'- -GAGUACGG-GAGCUGG---AGCa--CGUCA--AG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 28336 | 0.71 | 0.336326 |
Target: 5'- aCUCgAUGgCCUUGGCCUCGUcggGCAGcgCg -3' miRNA: 3'- -GAG-UACgGGAGCUGGAGCA---CGUCaaG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 3094 | 0.71 | 0.339683 |
Target: 5'- aCUUGUGCUguCUCGACCUgaugcggcgucauagCGUGCGGUUUc -3' miRNA: 3'- -GAGUACGG--GAGCUGGA---------------GCACGUCAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 32151 | 0.71 | 0.344765 |
Target: 5'- cCUCAagGCCgaaCUCGACCUUGccaaGCAGUUCc -3' miRNA: 3'- -GAGUa-CGG---GAGCUGGAGCa---CGUCAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 10760 | 0.7 | 0.427361 |
Target: 5'- ----cGCgCUCGACUUCGgcccGCAGUUCu -3' miRNA: 3'- gaguaCGgGAGCUGGAGCa---CGUCAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 18925 | 0.68 | 0.542417 |
Target: 5'- gUCcgGCCCgaccgagggUCG-CCUgGUGCAGUUg -3' miRNA: 3'- gAGuaCGGG---------AGCuGGAgCACGUCAAg -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 7355 | 0.68 | 0.542417 |
Target: 5'- ----cGgCCUCGACCUCG-GCGucGUUCg -3' miRNA: 3'- gaguaCgGGAGCUGGAGCaCGU--CAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 23491 | 0.67 | 0.609428 |
Target: 5'- -cCGUGggaaUCUCGACCUgCG-GCAGUUCc -3' miRNA: 3'- gaGUACg---GGAGCUGGA-GCaCGUCAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 26455 | 0.67 | 0.575703 |
Target: 5'- --gAUGCgCUCGACCUCGgcauccGuCAGUUUg -3' miRNA: 3'- gagUACGgGAGCUGGAGCa-----C-GUCAAG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 20196 | 0.66 | 0.643337 |
Target: 5'- gUCGUcGCCCUCGGUCUUG-GCAGcgCc -3' miRNA: 3'- gAGUA-CGGGAGCUGGAGCaCGUCaaG- -5' |
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26762 | 5' | -54.9 | NC_005808.1 | + | 7260 | 0.66 | 0.63203 |
Target: 5'- --gAUGUCgC-CGGCCUUGUGCAGcUUCu -3' miRNA: 3'- gagUACGG-GaGCUGGAGCACGUC-AAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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