Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26768 | 3' | -64.4 | NC_005808.1 | + | 15099 | 0.69 | 0.128855 |
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Target: 5'- -aGCUGGcGGCCgagGCCgCCGGCauCCUCg -3' miRNA: 3'- cgCGGCCaCCGGaa-CGG-GGUCG--GGAG- -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 16518 | 0.7 | 0.118794 |
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Target: 5'- gGUGCCGGUGGUCUgGCCgauaUAGCCgUa -3' miRNA: 3'- -CGCGGCCACCGGAaCGGg---GUCGGgAg -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 39768 | 0.7 | 0.115606 |
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Target: 5'- gGCGCCGcugcgcGUGGCCgcgaGCaCCUGGCCCg- -3' miRNA: 3'- -CGCGGC------CACCGGaa--CG-GGGUCGGGag -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 36215 | 0.7 | 0.106224 |
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Target: 5'- gGCGCCGcGgcGGCCgaccUgguguaacccagcGCCCCGGCCUUCg -3' miRNA: 3'- -CGCGGC-Ca-CCGGa---A-------------CGGGGUCGGGAG- -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 27435 | 0.71 | 0.100832 |
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Target: 5'- cGCGcCCGGcGGCCgcagGCCCUGGCgCUg -3' miRNA: 3'- -CGC-GGCCaCCGGaa--CGGGGUCGgGAg -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 31628 | 0.71 | 0.098099 |
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Target: 5'- -gGCCgaacaGGUGGCCgcagUGuCCCCGGCUgUCg -3' miRNA: 3'- cgCGG-----CCACCGGa---AC-GGGGUCGGgAG- -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 17292 | 0.71 | 0.095436 |
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Target: 5'- aGCGCCGGUGGCagggccgGCCgCAcGaCCUCa -3' miRNA: 3'- -CGCGGCCACCGgaa----CGGgGU-CgGGAG- -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 8461 | 0.72 | 0.080842 |
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Target: 5'- gGCGUCgaGGUGGCCUgcgaUGCCgCAGCCgaCg -3' miRNA: 3'- -CGCGG--CCACCGGA----ACGGgGUCGGgaG- -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 27499 | 0.74 | 0.054641 |
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Target: 5'- aUGCCGGcGGCCUcgGCCgCCAGCUuCUCg -3' miRNA: 3'- cGCGGCCaCCGGAa-CGG-GGUCGG-GAG- -5' |
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| 26768 | 3' | -64.4 | NC_005808.1 | + | 29362 | 1.11 | 0.00006 |
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Target: 5'- cGCGCCGGUGGCCUUGCCCCAGCCCUCg -3' miRNA: 3'- -CGCGGCCACCGGAACGGGGUCGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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