Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2677 | 3' | -48.4 | NC_001491.2 | + | 65087 | 0.66 | 0.999274 |
Target: 5'- gGCUUcUAUGUGCGAUGccgcaguaacGGCGCucugGGAa -3' miRNA: 3'- -UGAAuGUAUACGCUGC----------UCGUGua--CCU- -5' |
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2677 | 3' | -48.4 | NC_001491.2 | + | 146691 | 0.66 | 0.998374 |
Target: 5'- aGCggUACAcccaGCGACG-GUGCGUGGAg -3' miRNA: 3'- -UGa-AUGUaua-CGCUGCuCGUGUACCU- -5' |
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2677 | 3' | -48.4 | NC_001491.2 | + | 146252 | 0.7 | 0.983771 |
Target: 5'- --gUACGacUGCGACGAGgACggGGAg -3' miRNA: 3'- ugaAUGUauACGCUGCUCgUGuaCCU- -5' |
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2677 | 3' | -48.4 | NC_001491.2 | + | 148698 | 0.7 | 0.983771 |
Target: 5'- gGCgccccCAUuggGCGGCGgugGGCACAUGGAg -3' miRNA: 3'- -UGaau--GUAua-CGCUGC---UCGUGUACCU- -5' |
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2677 | 3' | -48.4 | NC_001491.2 | + | 66039 | 1.1 | 0.012453 |
Target: 5'- cACUUACAUAUGCGACGAGCACAUGGAg -3' miRNA: 3'- -UGAAUGUAUACGCUGCUCGUGUACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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