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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2677 | 5' | -56.7 | NC_001491.2 | + | 52876 | 0.72 | 0.545269 |
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Target: 5'- cGGCGCugGC-AACGCgCGCGGUGUu-- -3' miRNA: 3'- -CCGCGugUGuUUGCG-GUGCCGCAcga -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 80339 | 0.72 | 0.545269 |
|
Target: 5'- uGGCaaGCGC-CAAGCGaaACGGCGUGUa -3' miRNA: 3'- -CCG--CGUGuGUUUGCggUGCCGCACGa -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 10508 | 0.74 | 0.449646 |
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Target: 5'- cGC-CACGCAGcCGCCGCGGCGUaccuGCg -3' miRNA: 3'- cCGcGUGUGUUuGCGGUGCCGCA----CGa -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 10041 | 0.75 | 0.391835 |
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Target: 5'- cGGCGCACGCGGucucgcugccgcGCuccgugggggacuuuGCCGCGGuCGUGCg -3' miRNA: 3'- -CCGCGUGUGUU------------UG---------------CGGUGCC-GCACGa -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 82094 | 0.75 | 0.388489 |
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Target: 5'- gGGCGCGC-UAAACgGCCAUGGCGUuCUa -3' miRNA: 3'- -CCGCGUGuGUUUG-CGGUGCCGCAcGA- -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 12551 | 0.76 | 0.356082 |
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Target: 5'- gGGCGCgggagcGCGCGAGCGCCGCcucggGGCGcGCa -3' miRNA: 3'- -CCGCG------UGUGUUUGCGGUG-----CCGCaCGa -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 28537 | 0.78 | 0.277077 |
|
Target: 5'- aGGCGguuCACAUcgGGGCGCCACGGCGggGCg -3' miRNA: 3'- -CCGC---GUGUG--UUUGCGGUGCCGCa-CGa -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 5636 | 0.79 | 0.246061 |
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Target: 5'- uGCGCugGCug--GCCAUGGCGUGCUa -3' miRNA: 3'- cCGCGugUGuuugCGGUGCCGCACGA- -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 26713 | 0.87 | 0.073845 |
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Target: 5'- gGGCGCGCAgGuACGCCGCGGCG-GCUg -3' miRNA: 3'- -CCGCGUGUgUuUGCGGUGCCGCaCGA- -5' |
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| 2677 | 5' | -56.7 | NC_001491.2 | + | 66074 | 1.09 | 0.002327 |
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Target: 5'- aGGCGCACACAAACGCCACGGCGUGCUc -3' miRNA: 3'- -CCGCGUGUGUUUGCGGUGCCGCACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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