Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26773 | 5' | -53.1 | NC_005808.1 | + | 32916 | 0.66 | 0.74196 |
Target: 5'- cGGCCACGgccugcacggcGGCuguucgGCCAGggcGACcacgGCUGCGu -3' miRNA: 3'- -CCGGUGU-----------UCG------UGGUCa--UUGa---CGACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 40832 | 0.66 | 0.74196 |
Target: 5'- cGGCC-CGAcGCGCCAGgcGCagGCcgGCc -3' miRNA: 3'- -CCGGuGUU-CGUGGUCauUGa-CGa-CGu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 24897 | 0.66 | 0.74196 |
Target: 5'- cGGUCACGcGCGCCGGaaugAACgUGC-GCGa -3' miRNA: 3'- -CCGGUGUuCGUGGUCa---UUG-ACGaCGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 10787 | 0.66 | 0.74196 |
Target: 5'- cGGCCugGGaUugCAuGU-ACUGCUGCGc -3' miRNA: 3'- -CCGGugUUcGugGU-CAuUGACGACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 22302 | 0.66 | 0.730912 |
Target: 5'- cGGCCGcCGAGCaACCcgcuACUGgUGCGg -3' miRNA: 3'- -CCGGU-GUUCG-UGGucauUGACgACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 37793 | 0.66 | 0.729801 |
Target: 5'- cGGCCAgCAaucccgcAGCGCCAGccGGCgUGCUGg- -3' miRNA: 3'- -CCGGU-GU-------UCGUGGUCa-UUG-ACGACgu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 34834 | 0.66 | 0.719754 |
Target: 5'- cGGCgCAgAucGCGCCGGUGcugcaaucGCUGaCUGCGc -3' miRNA: 3'- -CCG-GUgUu-CGUGGUCAU--------UGAC-GACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 39782 | 0.66 | 0.70963 |
Target: 5'- uGGCCGCGAGCACCuGgcccGACgaaucgacgaaguggGC-GCAc -3' miRNA: 3'- -CCGGUGUUCGUGGuCa---UUGa--------------CGaCGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 34355 | 0.66 | 0.7085 |
Target: 5'- gGGCCGCuugAAGCGCa---AGCUGCUGaCGg -3' miRNA: 3'- -CCGGUG---UUCGUGgucaUUGACGAC-GU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 9570 | 0.66 | 0.7085 |
Target: 5'- cGGUgGCGAGCAuCCGGUcgAACacGUUGCGc -3' miRNA: 3'- -CCGgUGUUCGU-GGUCA--UUGa-CGACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 25369 | 0.66 | 0.7085 |
Target: 5'- cGGCCaccggGCAGGCGCCAGaAGCccuuUUGCGa -3' miRNA: 3'- -CCGG-----UGUUCGUGGUCaUUGac--GACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 1059 | 0.66 | 0.7085 |
Target: 5'- cGCCGCGaaccGGCGuCCAGUAACgcgcGCgGUAg -3' miRNA: 3'- cCGGUGU----UCGU-GGUCAUUGa---CGaCGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 41622 | 0.66 | 0.7085 |
Target: 5'- cGGCCA--GGCgGCCaAGUGGCUgcaaGCUGCc -3' miRNA: 3'- -CCGGUguUCG-UGG-UCAUUGA----CGACGu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 19466 | 0.66 | 0.701707 |
Target: 5'- cGCCAuaguucagcuugcgcCAGGUGCCGGUGggcagGCcGCUGCGa -3' miRNA: 3'- cCGGU---------------GUUCGUGGUCAU-----UGaCGACGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 18383 | 0.66 | 0.697164 |
Target: 5'- uGGCCuuGCcgauGGCGCCGGUGGCgGCcacgucgGCGa -3' miRNA: 3'- -CCGG--UGu---UCGUGGUCAUUGaCGa------CGU- -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 27700 | 0.66 | 0.697164 |
Target: 5'- aGGCCGC--GCGCCGGcAGCgGCUcGCc -3' miRNA: 3'- -CCGGUGuuCGUGGUCaUUGaCGA-CGu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 10308 | 0.67 | 0.685758 |
Target: 5'- aGGUCGCGcaacGCGCUGGUguuuuccugGAUUGCUGCu -3' miRNA: 3'- -CCGGUGUu---CGUGGUCA---------UUGACGACGu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 7256 | 0.67 | 0.685758 |
Target: 5'- cGGCCGCccAGguCUug-AACUGCUGCu -3' miRNA: 3'- -CCGGUGu-UCguGGucaUUGACGACGu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 4950 | 0.67 | 0.674296 |
Target: 5'- cGCCACGAuGCAgCCGcGUAucgcGCUGCUGg- -3' miRNA: 3'- cCGGUGUU-CGU-GGU-CAU----UGACGACgu -5' |
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26773 | 5' | -53.1 | NC_005808.1 | + | 10894 | 0.67 | 0.639693 |
Target: 5'- cGCgGCGGGCuggUCAGgcGgUGCUGCAg -3' miRNA: 3'- cCGgUGUUCGu--GGUCauUgACGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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