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miRNA: 3'- uUUGUGGUCgCGCaa-----CGCGCUGGagu -5'
miRNA: 3'- uuUGUG-GUCG--CGCaaCGCGCUGGa--GU- -5'
miRNA: 3'- uuugugGUCGCGCA--------ACGcGCUGGAGU- -5'
miRNA: 3'- uUUGU----GGUCGCGCaACGCGcuGGAGU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 26777 | 3' | -56 | NC_005808.1 | + | 29462 | 0.66 | 0.549525 |
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Target: 5'- uGGCACCAG-GCGacgaagcGCGCGGCCa-- -3' miRNA: 3'- uUUGUGGUCgCGCaa-----CGCGCUGGagu -5' |
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| 26777 | 3' | -56 | NC_005808.1 | + | 2231 | 0.66 | 0.560617 |
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Target: 5'- -cACGCgCAGCagGCGgcGCGUGGCCgcgCAa -3' miRNA: 3'- uuUGUG-GUCG--CGCaaCGCGCUGGa--GU- -5' |
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| 26777 | 3' | -56 | NC_005808.1 | + | 12521 | 0.66 | 0.560617 |
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Target: 5'- -----aCAGCGCGUcgaggugcUGCuCGACCUCGg -3' miRNA: 3'- uuugugGUCGCGCA--------ACGcGCUGGAGU- -5' |
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| 26777 | 3' | -56 | NC_005808.1 | + | 28747 | 0.66 | 0.560617 |
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Target: 5'- uGGCAucguCCAGCGCGgUGCGCu-UCUCGg -3' miRNA: 3'- uUUGU----GGUCGCGCaACGCGcuGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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