miRNA: 3'- uUUGuGGUCGCGCaACGCGC-UGGAgu -5'
miRNA: 3'- -UUUGUGGUCGCGCaACGcGCUGgAGU- -5'
miRNA: 3'- -UUUGuGGUCGCGC---AACGC-GCUgGAGU- -5'
miRNA: 3'- uUUGu---GGUCGCGc--AACGCGcUGGAGU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26777 | 3' | -56 | NC_005808.1 | + | 2574 | 0.68 | 0.46387 |
|
Target: 5'- cGGC-CCAGCGCG-UGCGCGcGCUUg- -3' miRNA: 3'- uUUGuGGUCGCGCaACGCGC-UGGAgu -5' |
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| 26777 | 3' | -56 | NC_005808.1 | + | 8442 | 0.68 | 0.46387 |
|
Target: 5'- gGAACAUCGGCGCGaUGU-CGGCgUCGa -3' miRNA: 3'- -UUUGUGGUCGCGCaACGcGCUGgAGU- -5' |
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| 26777 | 3' | -56 | NC_005808.1 | + | 21218 | 0.68 | 0.46387 |
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Target: 5'- cAGGC-CCAGCGCGccaUUGCGaCGAaCUCGg -3' miRNA: 3'- -UUUGuGGUCGCGC---AACGC-GCUgGAGU- -5' |
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| 26777 | 3' | -56 | NC_005808.1 | + | 3838 | 0.67 | 0.474217 |
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Target: 5'- cGACcaauCCAGCGCacuUUGCGC-ACCUCGc -3' miRNA: 3'- uUUGu---GGUCGCGc--AACGCGcUGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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