Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2678 | 3' | -53.3 | NC_001491.2 | + | 53186 | 0.66 | 0.967689 |
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Target: 5'- gCGGCAuCAAGCGgaGuCUUGCAGguGCCa -3' miRNA: 3'- aGCCGU-GUUCGCg-C-GAGUGUUauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 34181 | 0.66 | 0.967689 |
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Target: 5'- gCGGCGgAGGCGCcgggGCU-ACGAccGCCa -3' miRNA: 3'- aGCCGUgUUCGCG----CGAgUGUUauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1967 | 0.66 | 0.967689 |
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Target: 5'- aCGGCGCAGGCGa-CcCGCGG-AGUCa -3' miRNA: 3'- aGCCGUGUUCGCgcGaGUGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 69550 | 0.66 | 0.970445 |
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Target: 5'- cUCGGgGCAGGCaaagagaGCGUUgACAggGGCg -3' miRNA: 3'- -AGCCgUGUUCG-------CGCGAgUGUuaUCGg -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 84216 | 0.66 | 0.970741 |
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Target: 5'- -aGGCACcuccauacugGAGCGUGCcaCACAGU-GCUa -3' miRNA: 3'- agCCGUG----------UUCGCGCGa-GUGUUAuCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 137042 | 0.66 | 0.970741 |
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Target: 5'- -aGGCG-AGGCGCGCUguuugggagCGCAGUccuguuGCCg -3' miRNA: 3'- agCCGUgUUCGCGCGA---------GUGUUAu-----CGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 47119 | 0.66 | 0.970741 |
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Target: 5'- cCGGUGCAcgccccAGCagGCGCUC-CAG-AGCCc -3' miRNA: 3'- aGCCGUGU------UCG--CGCGAGuGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 62235 | 0.66 | 0.970741 |
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Target: 5'- aUCGaaaaGCACAAuCGUGUUCGCAucUAGUCa -3' miRNA: 3'- -AGC----CGUGUUcGCGCGAGUGUu-AUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 128157 | 0.66 | 0.970741 |
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Target: 5'- cUGGUACAGGgaCGaaguaucauuUGCUCGCA-UAGCCa -3' miRNA: 3'- aGCCGUGUUC--GC----------GCGAGUGUuAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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