Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2678 | 3' | -53.3 | NC_001491.2 | + | 1936 | 0.72 | 0.728174 |
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Target: 5'- gCGGCugAGGUgGCGCUCcCGG-GGCCa -3' miRNA: 3'- aGCCGugUUCG-CGCGAGuGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 102778 | 0.73 | 0.687223 |
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Target: 5'- -gGGUGCGuuGCGCGCUCcaGCAAacauuUAGCCa -3' miRNA: 3'- agCCGUGUu-CGCGCGAG--UGUU-----AUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 50480 | 0.73 | 0.676841 |
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Target: 5'- -aGGCACGGGUGCuGCccaacuaCAUGAUGGCCa -3' miRNA: 3'- agCCGUGUUCGCG-CGa------GUGUUAUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 24679 | 0.73 | 0.676841 |
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Target: 5'- gCGGCGCucGCGCGCUCcCGc--GCCc -3' miRNA: 3'- aGCCGUGuuCGCGCGAGuGUuauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 135917 | 0.73 | 0.666421 |
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Target: 5'- -aGGUaguGCAAuaGCGCUaCGCAAUAGCCc -3' miRNA: 3'- agCCG---UGUUcgCGCGA-GUGUUAUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 25037 | 0.73 | 0.657018 |
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Target: 5'- cCGGCACcgaggucuuccgcuGCGCGCUCGCuc--GCCg -3' miRNA: 3'- aGCCGUGuu------------CGCGCGAGUGuuauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1859 | 0.76 | 0.531394 |
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Target: 5'- -gGGUACAGGCGCGCgaaCGCGGccucGGCCc -3' miRNA: 3'- agCCGUGUUCGCGCGa--GUGUUa---UCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 100424 | 0.77 | 0.480798 |
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Target: 5'- aUCGGCcCGAGCcCGCUCGCAgcaugucAUAGUCg -3' miRNA: 3'- -AGCCGuGUUCGcGCGAGUGU-------UAUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 65400 | 1.12 | 0.003093 |
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Target: 5'- gUCGGCACAAGCGCGCUCACAAUAGCCc -3' miRNA: 3'- -AGCCGUGUUCGCGCGAGUGUUAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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