Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2678 | 3' | -53.3 | NC_001491.2 | + | 128157 | 0.66 | 0.970741 |
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Target: 5'- cUGGUACAGGgaCGaaguaucauuUGCUCGCA-UAGCCa -3' miRNA: 3'- aGCCGUGUUC--GC----------GCGAGUGUuAUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 128563 | 0.66 | 0.960922 |
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Target: 5'- gCGGCACuGGCGCuggucguuCUCAUcacAGCCg -3' miRNA: 3'- aGCCGUGuUCGCGc-------GAGUGuuaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 130757 | 0.7 | 0.840174 |
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Target: 5'- -aGGCGCcAGUGCGCUuagaCGCGAUAGa- -3' miRNA: 3'- agCCGUGuUCGCGCGA----GUGUUAUCgg -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 132020 | 0.68 | 0.924321 |
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Target: 5'- gUCGGUAUaugGAGCgGCGCUacgacCGAUAGCa -3' miRNA: 3'- -AGCCGUG---UUCG-CGCGAgu---GUUAUCGg -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 135917 | 0.73 | 0.666421 |
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Target: 5'- -aGGUaguGCAAuaGCGCUaCGCAAUAGCCc -3' miRNA: 3'- agCCG---UGUUcgCGCGA-GUGUUAUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 137042 | 0.66 | 0.970741 |
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Target: 5'- -aGGCG-AGGCGCGCUguuugggagCGCAGUccuguuGCCg -3' miRNA: 3'- agCCGUgUUCGCGCGA---------GUGUUAu-----CGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 139643 | 0.67 | 0.944586 |
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Target: 5'- -gGGCAgAAGCGCaUUgACGGcAGCCa -3' miRNA: 3'- agCCGUgUUCGCGcGAgUGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 142323 | 0.66 | 0.964418 |
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Target: 5'- aUCGaCACGGGCGUuuaCUCGCAc-GGCCg -3' miRNA: 3'- -AGCcGUGUUCGCGc--GAGUGUuaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 146692 | 0.66 | 0.956422 |
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Target: 5'- gCGGUACAcccAGCGacggugcguggaGCUCugGGUcuGGCCc -3' miRNA: 3'- aGCCGUGU---UCGCg-----------CGAGugUUA--UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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