Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2678 | 3' | -53.3 | NC_001491.2 | + | 3399 | 0.71 | 0.77733 |
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Target: 5'- aCGGCGCGcaccgcgaGGCGCaGCUCGuCGG-AGCCc -3' miRNA: 3'- aGCCGUGU--------UCGCG-CGAGU-GUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 3350 | 0.68 | 0.918629 |
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Target: 5'- cUCGGCguuguagcGCAccAGCgGCGC-CACGGUccgGGCCa -3' miRNA: 3'- -AGCCG--------UGU--UCG-CGCGaGUGUUA---UCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 2987 | 0.66 | 0.958714 |
|
Target: 5'- gCGGCugGgcugcugcccccgggAGCGgGCguuugUCugGGUGGCCc -3' miRNA: 3'- aGCCGugU---------------UCGCgCG-----AGugUUAUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 2613 | 0.68 | 0.924321 |
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Target: 5'- --cGCGCAAGC-CGCgUCGCug-GGCCg -3' miRNA: 3'- agcCGUGUUCGcGCG-AGUGuuaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1967 | 0.66 | 0.967689 |
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Target: 5'- aCGGCGCAGGCGa-CcCGCGG-AGUCa -3' miRNA: 3'- aGCCGUGUUCGCgcGaGUGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1936 | 0.72 | 0.728174 |
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Target: 5'- gCGGCugAGGUgGCGCUCcCGG-GGCCa -3' miRNA: 3'- aGCCGugUUCG-CGCGAGuGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1859 | 0.76 | 0.531394 |
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Target: 5'- -gGGUACAGGCGCGCgaaCGCGGccucGGCCc -3' miRNA: 3'- agCCGUGUUCGCGCGa--GUGUUa---UCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1774 | 0.67 | 0.949031 |
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Target: 5'- -gGGaaCGCGGGUGCGCUCGCc--GGCg -3' miRNA: 3'- agCC--GUGUUCGCGCGAGUGuuaUCGg -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1735 | 0.72 | 0.758008 |
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Target: 5'- gUCGG-GCAGGaCGCGCUgGCGGUacucccucggAGCCa -3' miRNA: 3'- -AGCCgUGUUC-GCGCGAgUGUUA----------UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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