Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2678 | 3' | -53.3 | NC_001491.2 | + | 109645 | 0.67 | 0.939894 |
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Target: 5'- aCGGCACGuGCGUuuGC-CGCcagGGCCu -3' miRNA: 3'- aGCCGUGUuCGCG--CGaGUGuuaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 39051 | 0.67 | 0.944586 |
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Target: 5'- gUCGGCGC-AGCGCcagGCUgAgAAgacGCCa -3' miRNA: 3'- -AGCCGUGuUCGCG---CGAgUgUUau-CGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 139643 | 0.67 | 0.944586 |
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Target: 5'- -gGGCAgAAGCGCaUUgACGGcAGCCa -3' miRNA: 3'- agCCGUgUUCGCGcGAgUGUUaUCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 1774 | 0.67 | 0.949031 |
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Target: 5'- -gGGaaCGCGGGUGCGCUCGCc--GGCg -3' miRNA: 3'- agCC--GUGUUCGCGCGAGUGuuaUCGg -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 10040 | 0.66 | 0.953233 |
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Target: 5'- gCGGCGCAcGCG-GuCUCGCu---GCCg -3' miRNA: 3'- aGCCGUGUuCGCgC-GAGUGuuauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 4005 | 0.66 | 0.953233 |
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Target: 5'- gUGGCAgggcccaGAGCGCGUUCcCGGccGCCa -3' miRNA: 3'- aGCCGUg------UUCGCGCGAGuGUUauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 33875 | 0.66 | 0.953233 |
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Target: 5'- gUGGCGCcgcuGGUGCGCU-ACAAc-GCCg -3' miRNA: 3'- aGCCGUGu---UCGCGCGAgUGUUauCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 146692 | 0.66 | 0.956422 |
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Target: 5'- gCGGUACAcccAGCGacggugcguggaGCUCugGGUcuGGCCc -3' miRNA: 3'- aGCCGUGU---UCGCg-----------CGAGugUUA--UCGG- -5' |
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| 2678 | 3' | -53.3 | NC_001491.2 | + | 128157 | 0.66 | 0.970741 |
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Target: 5'- cUGGUACAGGgaCGaaguaucauuUGCUCGCA-UAGCCa -3' miRNA: 3'- aGCCGUGUUC--GC----------GCGAGUGUuAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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