Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2678 | 5' | -48.3 | NC_001491.2 | + | 92614 | 0.66 | 0.998112 |
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Target: 5'- cUGUaagaCAGCAAAAACuGUGCGgCGg- -3' miRNA: 3'- uAUAg---GUCGUUUUUGuCACGCgGUau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 10438 | 0.66 | 0.998112 |
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Target: 5'- ---gCCGGCGGAcGCAGaGCGCUAc- -3' miRNA: 3'- uauaGGUCGUUUuUGUCaCGCGGUau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 134606 | 0.66 | 0.997723 |
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Target: 5'- -gGUCCcuC-GAAGCGGUGCGCCcgGa -3' miRNA: 3'- uaUAGGucGuUUUUGUCACGCGGuaU- -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 102825 | 0.67 | 0.995435 |
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Target: 5'- ----aCAGCAAcAGCAG-GCGCCGg- -3' miRNA: 3'- uauagGUCGUUuUUGUCaCGCGGUau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 98689 | 0.67 | 0.993731 |
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Target: 5'- ---aCCAGCAcgAAAGCAGUcUGCCAg- -3' miRNA: 3'- uauaGGUCGU--UUUUGUCAcGCGGUau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 2063 | 0.67 | 0.992704 |
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Target: 5'- -cGUCCAGCAccaGGAGCu-UGCGCCu-- -3' miRNA: 3'- uaUAGGUCGU---UUUUGucACGCGGuau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 130741 | 0.69 | 0.981292 |
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Target: 5'- -gAUCCcuGGgGAGGACAG-GCGCCAg- -3' miRNA: 3'- uaUAGG--UCgUUUUUGUCaCGCGGUau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 124485 | 0.74 | 0.853486 |
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Target: 5'- ---cCCAGUggAGAGACAGcGCGCCAUGg -3' miRNA: 3'- uauaGGUCG--UUUUUGUCaCGCGGUAU- -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 91136 | 0.77 | 0.682271 |
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Target: 5'- uUGUCCAGCAGGGGCAGcUGCcaugGCCAg- -3' miRNA: 3'- uAUAGGUCGUUUUUGUC-ACG----CGGUau -5' |
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| 2678 | 5' | -48.3 | NC_001491.2 | + | 65437 | 1.06 | 0.01778 |
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Target: 5'- cAUAUCCAGCAAAAACAGUGCGCCAUAa -3' miRNA: 3'- -UAUAGGUCGUUUUUGUCACGCGGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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