Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26780 | 5' | -55.8 | NC_005808.1 | + | 12190 | 0.66 | 0.604137 |
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Target: 5'- gAGAACgaaaccggcccgcgCUGCCUGCcccUGGUcGGGACGc -3' miRNA: 3'- -UCUUGa-------------GGCGGAUGuc-ACCA-CCCUGC- -5' |
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| 26780 | 5' | -55.8 | NC_005808.1 | + | 16141 | 0.67 | 0.544617 |
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Target: 5'- uAGGACUgCgGCCgcacgcugAUGGUGGUGGG-CGu -3' miRNA: 3'- -UCUUGA-GgCGGa-------UGUCACCACCCuGC- -5' |
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| 26780 | 5' | -55.8 | NC_005808.1 | + | 18274 | 0.67 | 0.533782 |
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Target: 5'- gAGAACUugccggCCGUCaGCGGgccGGUGGcGACGg -3' miRNA: 3'- -UCUUGA------GGCGGaUGUCa--CCACC-CUGC- -5' |
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| 26780 | 5' | -55.8 | NC_005808.1 | + | 7602 | 0.68 | 0.480905 |
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Target: 5'- cAGggUcugUCCGUCUGCcGUGGUgauggucagGGGGCGc -3' miRNA: 3'- -UCuuG---AGGCGGAUGuCACCA---------CCCUGC- -5' |
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| 26780 | 5' | -55.8 | NC_005808.1 | + | 42440 | 0.68 | 0.473702 |
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Target: 5'- cGGGGCgcaUCCGCCUGCGGggaagccUGGcgccacgccguagcuUGGGACa -3' miRNA: 3'- -UCUUG---AGGCGGAUGUC-------ACC---------------ACCCUGc -5' |
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| 26780 | 5' | -55.8 | NC_005808.1 | + | 30367 | 0.68 | 0.46047 |
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Target: 5'- gAGAAC-CCGCCgcUGCuGUagGGUGGGuCGg -3' miRNA: 3'- -UCUUGaGGCGG--AUGuCA--CCACCCuGC- -5' |
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| 26780 | 5' | -55.8 | NC_005808.1 | + | 33703 | 0.91 | 0.014656 |
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Target: 5'- gAGAACUCCGCaucCAGUGGUGGGACGc -3' miRNA: 3'- -UCUUGAGGCGgauGUCACCACCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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