Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26781 | 3' | -56.5 | NC_005808.1 | + | 28079 | 0.66 | 0.614161 |
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Target: 5'- cGGGC-AgGUGGCCGcGGcgucguauuccgucGCGGUGaUCGu -3' miRNA: 3'- -CCCGaUgCACCGGCuCC--------------UGCCAU-AGU- -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 13452 | 0.66 | 0.584457 |
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Target: 5'- -aGCUugGcGGCCucGGgcGCGGUGUCGg -3' miRNA: 3'- ccCGAugCaCCGGcuCC--UGCCAUAGU- -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 17922 | 0.66 | 0.562633 |
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Target: 5'- uGGGCguggACGUGGCCcgcGGCGGgcgCGa -3' miRNA: 3'- -CCCGa---UGCACCGGcucCUGCCauaGU- -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 29626 | 0.67 | 0.530338 |
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Target: 5'- cGGGCgcuUGUGGCCGGGcuuGAUGGg---- -3' miRNA: 3'- -CCCGau-GCACCGGCUC---CUGCCauagu -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 37859 | 0.67 | 0.509187 |
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Target: 5'- cGGC--CGUGGCCGAGaacaucGCGGUcAUCAu -3' miRNA: 3'- cCCGauGCACCGGCUCc-----UGCCA-UAGU- -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 23919 | 0.67 | 0.507091 |
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Target: 5'- aGGCccGCGUgguguaacaGGCCGGGGGCGGgcgccgcccccgAUCAc -3' miRNA: 3'- cCCGa-UGCA---------CCGGCUCCUGCCa-----------UAGU- -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 28078 | 0.68 | 0.488398 |
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Target: 5'- cGGCgACGUGGCUGAuuccuGGuauCGGUAUUc -3' miRNA: 3'- cCCGaUGCACCGGCU-----CCu--GCCAUAGu -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 24642 | 0.68 | 0.468017 |
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Target: 5'- uGGCgugccgGCG-GGCC-AGGAUGGUGUUg -3' miRNA: 3'- cCCGa-----UGCaCCGGcUCCUGCCAUAGu -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 5440 | 0.81 | 0.068064 |
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Target: 5'- uGGCgGCGUGGgCGAGGuCGGUAUCGa -3' miRNA: 3'- cCCGaUGCACCgGCUCCuGCCAUAGU- -5' |
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| 26781 | 3' | -56.5 | NC_005808.1 | + | 33757 | 0.91 | 0.012277 |
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Target: 5'- uGGGCUACGUcGG-CGAGGACGGUAUCAa -3' miRNA: 3'- -CCCGAUGCA-CCgGCUCCUGCCAUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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