Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26785 | 5' | -53.5 | NC_005808.1 | + | 13259 | 0.74 | 0.302881 |
Target: 5'- cGUGGUUCgAG-GACUUgGCCGGCGAc -3' miRNA: 3'- -UACUAAGgUCgCUGAAgCGGCUGCUc -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 40568 | 0.7 | 0.509999 |
Target: 5'- cGUG-UUCUAucgccugcGCGACUUCGCCaGCGAGc -3' miRNA: 3'- -UACuAAGGU--------CGCUGAAGCGGcUGCUC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 29234 | 0.68 | 0.633239 |
Target: 5'- cAUGGccuauUUCCAgaagcaauugcGCGACUUCGgCGGCGAc -3' miRNA: 3'- -UACU-----AAGGU-----------CGCUGAAGCgGCUGCUc -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 10037 | 0.67 | 0.68998 |
Target: 5'- cUGAUacgUCgGaGCGGCaUCGCCGGCGGc -3' miRNA: 3'- uACUA---AGgU-CGCUGaAGCGGCUGCUc -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 12327 | 0.66 | 0.712343 |
Target: 5'- ---uUUCCGGCGAC-UCGCgCGACcAGc -3' miRNA: 3'- uacuAAGGUCGCUGaAGCG-GCUGcUC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 14545 | 0.66 | 0.734367 |
Target: 5'- --uGUUCgAGCaGACcgUCGCCGACGuGu -3' miRNA: 3'- uacUAAGgUCG-CUGa-AGCGGCUGCuC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 33959 | 0.66 | 0.745221 |
Target: 5'- gAUGAaggCCGGCGACcUCGCCaccgcagcGGcCGAGa -3' miRNA: 3'- -UACUaa-GGUCGCUGaAGCGG--------CU-GCUC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 34353 | 1.08 | 0.001243 |
Target: 5'- gAUGAUUCCAGCGACUUCGCCGACGAGg -3' miRNA: 3'- -UACUAAGGUCGCUGAAGCGGCUGCUC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 37724 | 0.74 | 0.295165 |
Target: 5'- cAUGAUUgC-GUGGC-UCGCCGGCGAGg -3' miRNA: 3'- -UACUAAgGuCGCUGaAGCGGCUGCUC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 35453 | 0.68 | 0.621842 |
Target: 5'- cUGGUcaUCCAGgccgcagccgaCGACUUCGCgGACGuGa -3' miRNA: 3'- uACUA--AGGUC-----------GCUGAAGCGgCUGCuC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 5461 | 0.67 | 0.656019 |
Target: 5'- gAUG-UUCCcgccgGGCGGCgaggugUUGCCGAUGGGg -3' miRNA: 3'- -UACuAAGG-----UCGCUGa-----AGCGGCUGCUC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 712 | 0.66 | 0.701198 |
Target: 5'- -aGAUaCCgcccaggauaggGGCGGCUUUGCCGACa-- -3' miRNA: 3'- uaCUAaGG------------UCGCUGAAGCGGCUGcuc -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 18392 | 0.66 | 0.734368 |
Target: 5'- -cGAUggcgCCGGUGGCggccaCGUCGGCGAa -3' miRNA: 3'- uaCUAa---GGUCGCUGaa---GCGGCUGCUc -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 28666 | 0.66 | 0.734368 |
Target: 5'- -cGGUgCCGGCGGCcUCGCCaGCGcGg -3' miRNA: 3'- uaCUAaGGUCGCUGaAGCGGcUGCuC- -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 4818 | 0.66 | 0.734368 |
Target: 5'- -gGAUUgcuggCCGGCGACaggUCGCCGuaguCGAu -3' miRNA: 3'- uaCUAA-----GGUCGCUGa--AGCGGCu---GCUc -5' |
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26785 | 5' | -53.5 | NC_005808.1 | + | 32535 | 0.66 | 0.745221 |
Target: 5'- gGUGAUgCCGGCcagGAUgcugcCGCCGGCGAu -3' miRNA: 3'- -UACUAaGGUCG---CUGaa---GCGGCUGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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