Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26787 | 5' | -54.5 | NC_005808.1 | + | 4637 | 0.73 | 0.275802 |
Target: 5'- uGCU--GGGCCAcgcUGCGUCggccaucgcgUCAAGGUCg -3' miRNA: 3'- gCGAguUCCGGU---ACGCAG----------AGUUCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 14042 | 0.66 | 0.637157 |
Target: 5'- gCGCgccgCGAuGGCCG-GCGgccgCAAGGUCg -3' miRNA: 3'- -GCGa---GUU-CCGGUaCGCaga-GUUCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 35267 | 1.11 | 0.000586 |
Target: 5'- gCGCUCAAGGCCAUGCGUCUCAAGGUCg -3' miRNA: 3'- -GCGAGUUCCGGUACGCAGAGUUCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 32320 | 0.7 | 0.419773 |
Target: 5'- cCGCcaUCAAGGCCG-GCGUCcCGacaaccgccgcccAGGUCg -3' miRNA: 3'- -GCG--AGUUCCGGUaCGCAGaGU-------------UCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 15732 | 0.69 | 0.460955 |
Target: 5'- gGCgCGAGGCCAUGCG-CUCGAc--- -3' miRNA: 3'- gCGaGUUCCGGUACGCaGAGUUccag -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 8621 | 0.68 | 0.5031 |
Target: 5'- gCGCuUCucGGCCGcUGCGguggCGAGGUCg -3' miRNA: 3'- -GCG-AGuuCCGGU-ACGCaga-GUUCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 2588 | 0.68 | 0.524795 |
Target: 5'- aGCUugCAAGGCCA-GCagGUUggCGAGGUCg -3' miRNA: 3'- gCGA--GUUCCGGUaCG--CAGa-GUUCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 29060 | 0.68 | 0.556852 |
Target: 5'- gGCUCGuAGGCCAUcaGCGggUCAuccucgggcgccgAGGUCa -3' miRNA: 3'- gCGAGU-UCCGGUA--CGCagAGU-------------UCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 29564 | 0.67 | 0.603041 |
Target: 5'- uGCUCGGGGUaucGCGUC---AGGUCg -3' miRNA: 3'- gCGAGUUCCGguaCGCAGaguUCCAG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 35551 | 0.67 | 0.603041 |
Target: 5'- cCGCcCGAGGCCAUGCGccugCUgCAacAGG-Cg -3' miRNA: 3'- -GCGaGUUCCGGUACGCa---GA-GU--UCCaG- -5' |
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26787 | 5' | -54.5 | NC_005808.1 | + | 38204 | 0.66 | 0.637157 |
Target: 5'- aCGCUCAAGGCCcgGCG-Cgac-GG-Cg -3' miRNA: 3'- -GCGAGUUCCGGuaCGCaGaguuCCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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