Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26789 | 3' | -53.2 | NC_005808.1 | + | 1762 | 0.66 | 0.685758 |
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Target: 5'- uGGCcGGCCUGCgccuggCGCGUCGg- -3' miRNA: 3'- -CCGuUCGGACGaaagagGCGUAGCaa -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 30413 | 0.66 | 0.719754 |
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Target: 5'- cGCGGGCCgGUUUcgUUCuCGCAUCGg- -3' miRNA: 3'- cCGUUCGGaCGAAa-GAG-GCGUAGCaa -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 10003 | 0.66 | 0.730912 |
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Target: 5'- cGCGGGCCaGCUUggUCacaGCGUCGg- -3' miRNA: 3'- cCGUUCGGaCGAAagAGg--CGUAGCaa -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 35765 | 1.07 | 0.0013 |
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Target: 5'- gGGCAAGCCUGCUUUCUCCGCAUCGUUc -3' miRNA: 3'- -CCGUUCGGACGAAAGAGGCGUAGCAA- -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 30709 | 0.7 | 0.460588 |
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Target: 5'- uGGCGGGCaugGCUUUCUCCaucggaauGCGUCa-- -3' miRNA: 3'- -CCGUUCGga-CGAAAGAGG--------CGUAGcaa -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 30313 | 0.69 | 0.513288 |
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Target: 5'- uGCGAGCC-GCUUUgcguguaCUUCGCGUCGg- -3' miRNA: 3'- cCGUUCGGaCGAAA-------GAGGCGUAGCaa -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 9417 | 0.68 | 0.616567 |
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Target: 5'- cGCGGGCUUGCaggUCUUgaauaGCGUCGUUg -3' miRNA: 3'- cCGUUCGGACGaa-AGAGg----CGUAGCAA- -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 21187 | 0.67 | 0.628128 |
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Target: 5'- uGCGcGGCCUGCUgcuggUCUgCGCGUUGc- -3' miRNA: 3'- cCGU-UCGGACGAa----AGAgGCGUAGCaa -5' |
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| 26789 | 3' | -53.2 | NC_005808.1 | + | 26262 | 0.66 | 0.7085 |
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Target: 5'- gGGCAcccgccGGCCUGgUcggCUCgCGCAUCGc- -3' miRNA: 3'- -CCGU------UCGGACgAaa-GAG-GCGUAGCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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