Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2680 | 3' | -54.8 | NC_001491.2 | + | 104182 | 0.66 | 0.915583 |
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Target: 5'- uCGGAAUgUCcgcGGGcGCCUGCGaGGGc- -3' miRNA: 3'- -GCCUUAgAGa--CCCaCGGAUGC-CCUuu -5' |
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| 2680 | 3' | -54.8 | NC_001491.2 | + | 99729 | 0.67 | 0.890004 |
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Target: 5'- uGGAGg----GGG-GCCUAUGGGAGAa -3' miRNA: 3'- gCCUUagagaCCCaCGGAUGCCCUUU- -5' |
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| 2680 | 3' | -54.8 | NC_001491.2 | + | 134464 | 0.67 | 0.883014 |
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Target: 5'- gCGcGAGg-UCUGGGUGCUuguggcugUGCGGGGAc -3' miRNA: 3'- -GC-CUUagAGACCCACGG--------AUGCCCUUu -5' |
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| 2680 | 3' | -54.8 | NC_001491.2 | + | 11942 | 0.68 | 0.8528 |
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Target: 5'- aCGGcaccUCgCUGGGUGCg-ACGGGGAAg -3' miRNA: 3'- -GCCuu--AGaGACCCACGgaUGCCCUUU- -5' |
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| 2680 | 3' | -54.8 | NC_001491.2 | + | 5318 | 0.69 | 0.801435 |
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Target: 5'- cCGGAGcCgggCUGGGUGCCg--GGGAc- -3' miRNA: 3'- -GCCUUaGa--GACCCACGGaugCCCUuu -5' |
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| 2680 | 3' | -54.8 | NC_001491.2 | + | 63147 | 1.06 | 0.004863 |
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Target: 5'- gCGGAAUCUCUGGGUGCCUACGGGAAAg -3' miRNA: 3'- -GCCUUAGAGACCCACGGAUGCCCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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