Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2680 | 5' | -53.3 | NC_001491.2 | + | 23658 | 0.66 | 0.964372 |
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Target: 5'- --gCGgCCGCACCGCUGuagcugggauccaccGAGGGUGUGg -3' miRNA: 3'- gaaGUgGGCGUGGUGGU---------------CUCUCAUAU- -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 61404 | 0.67 | 0.937673 |
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Target: 5'- -cUC-CCCaGCGCCACCAGAccgucguuccugGGGUAg- -3' miRNA: 3'- gaAGuGGG-CGUGGUGGUCU------------CUCAUau -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 130150 | 0.67 | 0.937673 |
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Target: 5'- --aCGCCCgGCACgGCUggggcgcauguAGGGAGUGUAa -3' miRNA: 3'- gaaGUGGG-CGUGgUGG-----------UCUCUCAUAU- -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 32442 | 0.67 | 0.932585 |
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Target: 5'- --gCGCCCGCucccaGCCGCCcGGGAGg--- -3' miRNA: 3'- gaaGUGGGCG-----UGGUGGuCUCUCauau -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 150048 | 0.68 | 0.909693 |
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Target: 5'- ---gGCCCGCGCC-CgAGAGAGa--- -3' miRNA: 3'- gaagUGGGCGUGGuGgUCUCUCauau -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 64504 | 0.7 | 0.8181 |
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Target: 5'- ---gGCCUGCGCCGCCAcacgucucuGGGGGUGUc -3' miRNA: 3'- gaagUGGGCGUGGUGGU---------CUCUCAUAu -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 64361 | 0.7 | 0.799968 |
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Target: 5'- --aCGCCUGCAUCACgGGGGGGUu-- -3' miRNA: 3'- gaaGUGGGCGUGGUGgUCUCUCAuau -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 52674 | 0.72 | 0.741007 |
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Target: 5'- cCUUCACCCGCAgaCGCggcggguCGGAGGGUAa- -3' miRNA: 3'- -GAAGUGGGCGUg-GUG-------GUCUCUCAUau -5' |
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| 2680 | 5' | -53.3 | NC_001491.2 | + | 63182 | 1.06 | 0.006507 |
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Target: 5'- uCUUCACCCGCACCACCAGAGAGUAUAc -3' miRNA: 3'- -GAAGUGGGCGUGGUGGUCUCUCAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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