Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26801 | 5' | -62.8 | NC_005808.1 | + | 38223 | 1.1 | 0.000101 |
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Target: 5'- aCGGCGCCUGGCUGCGCAUCCGCCUGCc -3' miRNA: 3'- -GCCGCGGACCGACGCGUAGGCGGACG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 38746 | 0.69 | 0.171801 |
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Target: 5'- aCGGCGCCUGGUacaacuucgccgagGUcaugacucaccacgGCAagCGCCUGCc -3' miRNA: 3'- -GCCGCGGACCGa-------------CG--------------CGUagGCGGACG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 39071 | 0.67 | 0.216841 |
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Target: 5'- gCGGUGUgcGGCUGUaugucuauaaGUGUCCGCaCUGCa -3' miRNA: 3'- -GCCGCGgaCCGACG----------CGUAGGCG-GACG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 39767 | 0.75 | 0.06011 |
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Target: 5'- uGGCGCC--GCUGCGCGUggCCGCgaGCa -3' miRNA: 3'- gCCGCGGacCGACGCGUA--GGCGgaCG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 41239 | 0.66 | 0.265605 |
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Target: 5'- uCGGuCGCC-GGaUGCGCAaggCCGCCgaccacgGCu -3' miRNA: 3'- -GCC-GCGGaCCgACGCGUa--GGCGGa------CG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 41913 | 0.72 | 0.102321 |
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Target: 5'- gGGCGCCcGGCcgGCGCcgCCGUagGCg -3' miRNA: 3'- gCCGCGGaCCGa-CGCGuaGGCGgaCG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 42108 | 0.66 | 0.259054 |
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Target: 5'- gCGGCGCaguauGCUGCcgagGCAUCCGaCC-GCg -3' miRNA: 3'- -GCCGCGgac--CGACG----CGUAGGC-GGaCG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 42246 | 0.67 | 0.234165 |
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Target: 5'- uCGG-GCCUGGCggacggccugcUGCGCGacgccaCCGCCaGCc -3' miRNA: 3'- -GCCgCGGACCG-----------ACGCGUa-----GGCGGaCG- -5' |
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| 26801 | 5' | -62.8 | NC_005808.1 | + | 42423 | 0.82 | 0.017016 |
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Target: 5'- aCGGUGCCcaGCgacaccgggGCGCAUCCGCCUGCg -3' miRNA: 3'- -GCCGCGGacCGa--------CGCGUAGGCGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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