Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26803 | 3' | -51.6 | NC_005808.1 | + | 41107 | 0.68 | 0.749168 |
Target: 5'- aUCAAGUaCGUGAGCcgGuGGCGGAa-- -3' miRNA: 3'- -AGUUUAgGCGCUCGuaC-UCGCCUacg -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 38872 | 1.14 | 0.000747 |
Target: 5'- aUCAAAUCCGCGAGCAUGAGCGGAUGCg -3' miRNA: 3'- -AGUUUAGGCGCUCGUACUCGCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 35475 | 0.68 | 0.749168 |
Target: 5'- aCGAcUUCGCGGaCGUGAGCGGGg-- -3' miRNA: 3'- aGUUuAGGCGCUcGUACUCGCCUacg -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 33515 | 0.67 | 0.770638 |
Target: 5'- aCGGccUCGCG-GCcaGUGAGgGGAUGCa -3' miRNA: 3'- aGUUuaGGCGCuCG--UACUCgCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 31445 | 0.69 | 0.65912 |
Target: 5'- --cGAUCUGCG-GCGUGgucaucggaaAGUGGGUGCc -3' miRNA: 3'- aguUUAGGCGCuCGUAC----------UCGCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 31359 | 0.67 | 0.800627 |
Target: 5'- cUCAucgCCacgcuaGUGGGCGUGGGCGGGaacguggcggucaUGCg -3' miRNA: 3'- -AGUuuaGG------CGCUCGUACUCGCCU-------------ACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 28721 | 0.68 | 0.716018 |
Target: 5'- gUCGGcgCaCGCGAGgAUGAGCGccuugGCa -3' miRNA: 3'- -AGUUuaG-GCGCUCgUACUCGCcua--CG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 25100 | 0.7 | 0.613026 |
Target: 5'- cCGGcgCCGUGGGauacAGCGGGUGCu -3' miRNA: 3'- aGUUuaGGCGCUCguacUCGCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 24310 | 0.69 | 0.670604 |
Target: 5'- -gAGGUCgGCGAGCAgccGCGGGUcagGCg -3' miRNA: 3'- agUUUAGgCGCUCGUacuCGCCUA---CG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 16328 | 0.67 | 0.789427 |
Target: 5'- --cGAUgCGCGAGCcgaccaggccGGCGGGUGCc -3' miRNA: 3'- aguUUAgGCGCUCGuac-------UCGCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 14104 | 0.66 | 0.840185 |
Target: 5'- cCGAGUUCgGCaauGCc-GAGCGGAUGCu -3' miRNA: 3'- aGUUUAGG-CGcu-CGuaCUCGCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 12258 | 0.67 | 0.759975 |
Target: 5'- aCGGAUCgGCG-GUAguguccUGGGCGGGuUGCu -3' miRNA: 3'- aGUUUAGgCGCuCGU------ACUCGCCU-ACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 9727 | 0.67 | 0.759975 |
Target: 5'- cUCGAAgCCGCGGGCGaacUGcucguGGUGGgcGCg -3' miRNA: 3'- -AGUUUaGGCGCUCGU---AC-----UCGCCuaCG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 4358 | 0.67 | 0.79148 |
Target: 5'- -gGAAUCgGCcggacGGCA--GGCGGAUGCg -3' miRNA: 3'- agUUUAGgCGc----UCGUacUCGCCUACG- -5' |
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26803 | 3' | -51.6 | NC_005808.1 | + | 4207 | 0.66 | 0.840185 |
Target: 5'- -----gCCGCGAGCAcGucGCGGcuUGCg -3' miRNA: 3'- aguuuaGGCGCUCGUaCu-CGCCu-ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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